Study on The Method of Quantitative Analysis of Serum Ferritin and Soluble Transferrin Receptor with Protein Microarray Technology

Objective To establish and evaluate a protein serum ferritin （SF） and soluble transferrin receptor microarray method for combined measurement of （sTfR）. Methods Microarrayer was used to print both anti-SF antibodies I and anti-sTfR antibodies I on each protein microarray. Anti-SF antibodies II and a...

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Published in:Biomedical and environmental sciences 2012-08, Vol.25 (4), p.430-439
Main Authors: YIN, Ji Yong, SUN, Jing, HUANG, Jian, LI, Wen Xian, HUO, Jun Sheng
Format: Article
Language:English
Subjects:
Animals
Antibodies, Monoclonal - analysis
Combined measurement conditions
Ferritins - blood
Mice
Optimization
Protein Array Analysis - methods
Protein microarray
Rabbits
Receptors, Transferrin - blood
Serum ferritin
Soluble transferrin receptor
单克隆抗体
受体蛋白
可溶性
定量分析方法
芯片技术
蛋白质微阵列
血清铁蛋白
转铁蛋白受体
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container_title Biomedical and environmental sciences
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creator YIN, Ji Yong
SUN, Jing
HUANG, Jian
LI, Wen Xian
HUO, Jun Sheng
description Objective To establish and evaluate a protein serum ferritin （SF） and soluble transferrin receptor microarray method for combined measurement of （sTfR）. Methods Microarrayer was used to print both anti-SF antibodies I and anti-sTfR antibodies I on each protein microarray. Anti-SF antibodies II and anti-sTfR antibodies II were used as detection antibodies and goat antibodies coupled to Cy3 were used as antibodies Ill. The detection conditions of the quantitative analysis method for simultaneous measurement of SF and sTfR with protein microarray were optimized and evaluated. The protein microarray was compared with commercially available traditional tests with 26 serum samples. Results By comparison experiment, mouse monoclonal antibodies were chosen as the probes and contact printing was chosen as the printing method. The concentrations of SF and sTfR probes were 0.5 mg/mL and 0.5 mg/mL respectively, while those of SF and sTfR detection antibodies were 5 μg/mL and 0.36 μg/mL respectively. Intra- and inter-assay variability was between 3.26% and 18.38% for all tests. The regression coefficients comparing protein microarray with traditional test assays were better than 0.81 for SF and sTfR. Conclusion The present study has established a protein microarray method for combined measurement of SF and sTfR.
doi_str_mv 10.3967/0895-3988.2012.04.008
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Methods Microarrayer was used to print both anti-SF antibodies I and anti-sTfR antibodies I on each protein microarray. Anti-SF antibodies II and anti-sTfR antibodies II were used as detection antibodies and goat antibodies coupled to Cy3 were used as antibodies Ill. The detection conditions of the quantitative analysis method for simultaneous measurement of SF and sTfR with protein microarray were optimized and evaluated. The protein microarray was compared with commercially available traditional tests with 26 serum samples. Results By comparison experiment, mouse monoclonal antibodies were chosen as the probes and contact printing was chosen as the printing method. The concentrations of SF and sTfR probes were 0.5 mg/mL and 0.5 mg/mL respectively, while those of SF and sTfR detection antibodies were 5 μg/mL and 0.36 μg/mL respectively. Intra- and inter-assay variability was between 3.26% and 18.38% for all tests. The regression coefficients comparing protein microarray with traditional test assays were better than 0.81 for SF and sTfR. Conclusion The present study has established a protein microarray method for combined measurement of SF and sTfR.</description><identifier>ISSN: 0895-3988</identifier><identifier>EISSN: 2214-0190</identifier><identifier>DOI: 10.3967/0895-3988.2012.04.008</identifier><identifier>PMID: 23026523</identifier><language>eng</language><publisher>China: Elsevier B.V</publisher><subject>Animals ; Antibodies, Monoclonal - analysis ; Combined measurement conditions ; Ferritins - blood ; Mice ; Optimization ; Protein Array Analysis - methods ; Protein microarray ; Rabbits ; Receptors, Transferrin - blood ; Serum ferritin ; Soluble transferrin receptor ; 单克隆抗体 ; 受体蛋白 ; 可溶性 ; 定量分析方法 ; 芯片技术 ; 蛋白质微阵列 ; 血清铁蛋白 ; 转铁蛋白受体</subject><ispartof>Biomedical and environmental sciences, 2012-08, Vol.25 (4), p.430-439</ispartof><rights>2012 The Editorial Board of Biomedical and Environmental Sciences</rights><rights>Copyright © 2012 The Editorial Board of Biomedical and Environmental Sciences. 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Methods Microarrayer was used to print both anti-SF antibodies I and anti-sTfR antibodies I on each protein microarray. Anti-SF antibodies II and anti-sTfR antibodies II were used as detection antibodies and goat antibodies coupled to Cy3 were used as antibodies Ill. The detection conditions of the quantitative analysis method for simultaneous measurement of SF and sTfR with protein microarray were optimized and evaluated. The protein microarray was compared with commercially available traditional tests with 26 serum samples. Results By comparison experiment, mouse monoclonal antibodies were chosen as the probes and contact printing was chosen as the printing method. The concentrations of SF and sTfR probes were 0.5 mg/mL and 0.5 mg/mL respectively, while those of SF and sTfR detection antibodies were 5 μg/mL and 0.36 μg/mL respectively. Intra- and inter-assay variability was between 3.26% and 18.38% for all tests. 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identifier ISSN: 0895-3988
ispartof Biomedical and environmental sciences, 2012-08, Vol.25 (4), p.430-439
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2214-0190
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source ScienceDirect Journals
subjects Animals
Antibodies, Monoclonal - analysis
Combined measurement conditions
Ferritins - blood
Mice
Optimization
Protein Array Analysis - methods
Protein microarray
Rabbits
Receptors, Transferrin - blood
Serum ferritin
Soluble transferrin receptor
单克隆抗体
受体蛋白
可溶性
定量分析方法
芯片技术
蛋白质微阵列
血清铁蛋白
转铁蛋白受体
title Study on The Method of Quantitative Analysis of Serum Ferritin and Soluble Transferrin Receptor with Protein Microarray Technology
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