Immunotoxicological Evaluation of Wheat Genetically Modified with TaDREB4 Gene on BALB/c Mice

Objective To evaluate the immunotoxicological effects of genetically modified wheat with TaDREB4 gene in female BALB/c mice. Methods Female mice weighing 18-22 g were divided into five groups （10 mice/group）, which were set as negative control group, common wheat group, parental wheat group, genetic...

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Bibliographic Details
Published in:Biomedical and environmental sciences 2013-08, Vol.26 (8), p.663-670
Main Authors: LIANG, Chun Lai, ZHANG, Xiao Peng, SONG, Yan, JIA, Xu Dong
Format: Article
Language:English
Subjects:
Animals
Antibody-Producing Cells - immunology
BALB/c mice
Body Weight
Cytokines - blood
Female
Genes, Plant
Genetically modified wheat
Hemolysis
Hypersensitivity, Delayed
Immune System - drug effects
Immunoglobulins - blood
Immunotoxicological effects
Mice
Mice, Inbred BALB C
Organ Size
Phagocytosis
Plants, Genetically Modified - toxicity
Spleen - immunology
Thymus Gland - immunology
Triticum - genetics
基因改造
对照组
普通小麦
白细胞计数
血清免疫球蛋白
评价
转基因小麦
雌性小鼠
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Summary:Objective To evaluate the immunotoxicological effects of genetically modified wheat with TaDREB4 gene in female BALB/c mice. Methods Female mice weighing 18-22 g were divided into five groups （10 mice/group）, which were set as negative control group, common wheat group, parental wheat group, genetically modified wheat group and cyclophosphamide positive control group, respectively. Mice in negative control group and positive control group were fed with AIN93G diet, mice in common wheat group, non-genetically modified parental wheat group and genetically modified wheat group were fed with feedstuffs added corresponding wheat （the proportion is 76%} for 30 days, then body weight, absolute and relative weight of spleen and thymus, white blood cell count, histological examination of immune organ, peripheral blood lymphocytes phenotyping, serum cytokine, serum immunoglobulin, antibody plaque-forming cell, serum half hemolysis value, mitogen-induced splenocyte proliferation, delayed-type hypersensitivity reaction and phagocytic activities of phagocytes were detected. Results No immunotoxicological effects related to the consumption of the genetically modified wheat were observed in BALB/c mice when compared with parental wheat group, common wheat group and negative control group. Conclusion From the immunotoxicological point of view, results from this study demonstrate that genetically modified wheat with TaDREB4 gene is as safe as the parental wheat.
ISSN:0895-3988
2214-0190
DOI:10.3967/0895-3988.2013.08.005