N-hexane Alters the Maturation of Oocytes and Induces Apoptosis in Mice

Objective This study was aimed to determine the effects of n-hexane on the maturation of mouse oocytes. Methods Cell culture was used to observe the maturation of mouse oocytes and CLSM was employed to determine their apoptosis. Results Germinal vesicle breakdown （GVBD） and extrusion of the first po...

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Bibliographic Details
Published in:Biomedical and environmental sciences 2013-09, Vol.26 (9), p.735-741
Main Authors: LIU, Jin, HUANG, Lei, SUN, Yan, LI, Yu Chen, ZHU, Jian Lin, WANG, Wen Xiang, ZHANG, Wen Chang
Format: Article
Language:English
Subjects:
Animals
Apoptosis - drug effects
Environmental Pollutants - toxicity
Female
Fertilization
Fertilization - drug effects
Hexanes - toxicity
Male
Maturation
Membrane Potential, Mitochondrial - drug effects
Mice
Mice, Inbred ICR
Mitochondrial membrane potential
N-hexane
Oocytes - drug effects
Oocytes - growth & development
体内
卵母细胞成熟
小鼠卵母细胞
正己烷
激光共聚焦显微镜
线粒体膜电位
细胞凋亡
诱导
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Summary:Objective This study was aimed to determine the effects of n-hexane on the maturation of mouse oocytes. Methods Cell culture was used to observe the maturation of mouse oocytes and CLSM was employed to determine their apoptosis. Results Germinal vesicle breakdown （GVBD） and extrusion of the first polar body in mouse oocytes were significantly inhibited by n-hexane. After fertilization, the number of eggs in the mouse was significantly reduced by n-hexane. Mitochondrial membrane potentials （A~Um） were altered in mouse oocytes that were leading to apoptosis of the oocytes. Conclusion N-hexane might have affected the maturation of oocytes, causing alteration of ~qJm and leading to apoptosis which maybe one of the most imn（~rt~nt rnpch~ni~nn~
ISSN:0895-3988
2214-0190
DOI:10.3967/0895-3988.2013.09.004