Prevention of H2O2 Induced Oxidative Damages of Rat Testis by Thymus algeriensis

• Published in: Biomedical and environmental sciences 2016-04, Vol.29 (4), p.275-285
• Main Authors: Guesmi, Fatma, Beghalem, Hamida, Tyagi, Amit K, Ali, Manel Ben, Mouhoub, Ramla Ben, Bellamine, Houda, Landoulsi, Ahmed
• Format: Article
• Language: English
• Subjects: Animals; Antioxidants; H2O2; Hydrogen peroxide; Hydrogen Peroxide - toxicity; Infertility; Male; Oxidative Stress; Plant Extracts - pharmacology; Rats; Rats, Wistar; TEO; Testis; Testis - drug effects; Thymus Plant - chemistry; 大鼠; 抗氧化酶活性; 氧化损伤; 睾丸组织; 胸腺; 诱导; 过氧化氢
• ISSN: 0895-3988; 2214-0190
• DOI: 10.3967/bes2016.035

Objective We evaluate the effects of Thymus algeriensis （TEO） against hydrogen peroxide （H202） toxicity on body and testis weight, testis sperm count, testis lipid peroxidation, and antioxidant enzyme activities in rats. Methods Rats were treated with low （LD） and high dose （HD） of H202 （0.1 and 1 mmol/L） in the presence or absence of TEO （150 mg/kg）. Results The results exhibited a significant decrease in body weight and testis weight, in total sperm number decrease （P〈0.05）, sperm motility and percentage of sperm viability, leading to complete arrest, in sperm flagellar beat frequency by the gavage of 1 mmol/L H202 compared to controls. The administration of H2O2 resulted in a significant reduction in testis GSH, GPx, CAT, SOD, and GST activity and significant increase （P〈0.05） in MDA concentration compared with the untreated control animals. TEO pre-treatment protected testis from the H2O2 generated oxidative stress. These results were confirmed by histological architecture examinations. Conclusion H2O2 has the ability to alter the sperm function, characteristics and development of testis. However, TEO is an efficient natural agent, which can prevent the testis from H2O2-induced oxidative damage in rats.