Development of an Internally Controlled Reverse Transcription Recombinase-aided Amplification Assay for the Rapid and Visual Detection of West Nile Virus

West Nile virus (WNV) causes West Nile fever and West Nile encephalitis. Because infection by WNV creates serious public health problems, its simple, rapid, and visual detection is very important in clinical practice, especially in resource-limited laboratories. We have developed a rapid, specific,...

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Bibliographic Details
Published in:Biomedical and environmental sciences 2019-12, Vol.32 (12), p.926-929
Main Authors: FAN, Guo Hao, SHEN, Xin Xin, LI, Fan, LI, Xin Na, BAI, Xue Ding, ZHANG, Rui Qing, WANG, Rui Huan, LEI, Wen Wen, WANG, Huan Yu, MA, Xue Jun, WU, Gui Zhen
Format: Article
Language:English
Subjects:
Nucleic Acid Amplification Techniques - methods
Recombinases - metabolism
Reverse Transcription
Time Factors
West Nile virus - genetics
West Nile virus - isolation & purification
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Summary:West Nile virus (WNV) causes West Nile fever and West Nile encephalitis. Because infection by WNV creates serious public health problems, its simple, rapid, and visual detection is very important in clinical practice, especially in resource-limited laboratories. We have developed a rapid, specific, and highly sensitive internally controlled reverse transcription recombinase-aided amplification (RTRAA) assay to detect WNV, using both real-time fluorescence and the lateral flow dipstick (LFD) at 39.0 °C for 30 min. The analytical sensitivity of the RT-RAA assay was 10 plasmid copies and 1.6 pfu per reaction with real-time fluorescence, and 1,000 plasmid copies per reaction with the LFD. No crossreaction with other control viruses was observed. Compared with the RT-qPCR assay, the RT-RAA assay demonstrated 100% sensitivity and 100% specificity for WNV.
ISSN:0895-3988
2214-0190
DOI:10.3967/bes2019.116