Genetic Characteristics of Lipooligosaccharide and Capsular Polysaccharide of Campylobacter jejuni from Different Sources in China

To determine the distribution of two important virulence factors [lipooligosaccharide (LOS) and capsular polysaccharide (CPS)] in Campylobacter jejuni (C. jejuni) isolated from different sources in China and to develop a rapid screening method for Guillain–Barré syndrome (GBS)-associated strains. Wh...

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Published in:Biomedical and environmental sciences 2022-12, Vol.35 (12), p.1106-1114
Main Authors: WANG, Jia Qi, CHEN, Xiao Li, ZHOU, Gui Lan, WANG, Hai Rui, GU, Yi Xin, ZHANG, Jian Zhong, SHAO, Zhu Jun, ZHANG, Mao Jun
Format: Article
Language:English
Subjects:
Campylobacter Infections
Campylobacter jejuni
Campylobacter jejuni - genetics
Capsular polysaccharide
China - epidemiology
Humans
Lipooligosaccharide
Lipopolysaccharides
Multiplex real-time PCR
Virulence Factors
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Summary:To determine the distribution of two important virulence factors [lipooligosaccharide (LOS) and capsular polysaccharide (CPS)] in Campylobacter jejuni (C. jejuni) isolated from different sources in China and to develop a rapid screening method for Guillain–Barré syndrome (GBS)-associated strains. Whole-genome sequencing was carried out for 494 C. jejuni strains. The OrthoMCL software was used to define the LOS/CPS gene clusters. CPS genotyping was performed with serotype-specific sequence alignment using the BLAST software. Real-time Polymerase chain reaction (PCR) was developed with the unique sequences of specific CPS types. Nine novel and 29 previously confirmed LOS classes were identified. LOS classes A, B, and C were the most common (48.2%, 238/494) among the 494 strains. Twenty-six capsular types were identified in 448 strains. HS2, HS4c, HS5/31, HS19, and HS8/17 were the most frequent CPS genotypes (58.7%, 263/448). Strains of 17 CPS genotypes (strain number > 5) had one or two prevalent LOS classes (P < 0.05). Multiplex real-time PCR for rapid identification of HS2, HS19, and HS41 was developed and validated with strains of known serotypes. Our results describe the genetic characteristics of the important virulence factors in C. jejuni strains in China. The multiplex real-time PCR developed in this study will facilitate enhanced surveillance of GBS-associated strains in China.
ISSN:0895-3988
2214-0190
DOI:10.3967/bes2022.140