Covalent immobilization and characterization of Rhizopus oryzae lipase on core-shell cobalt ferrite nanoparticles for biodiesel production

[Display omitted] Rhizopus oryzae lipase (ROL) was immobilized on the surface of silica coated amino modified CoFe2O4 nanoparticles and applied for biodiesel production. The results indicated more affinity of the ROL toward its substrate upon immobilization, as revealed by a lower Km value for the i...

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Bibliographic Details
Published in:Chinese journal of chemical engineering 2021-09, Vol.37 (9), p.128-136
Main Authors: Ashkevarian, Saboura, Badraghi, Jalil, Mamashli, Fatemeh, Delavari, Behdad, Saboury, Ali Akbar
Format: Article
Language:English
Subjects:
Biocatalysis
Biodiesel
CoFe2O4 magnetic nanoparticles
Lipase immobilization
Protein stability
Steady state anisotropy
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Summary:[Display omitted] Rhizopus oryzae lipase (ROL) was immobilized on the surface of silica coated amino modified CoFe2O4 nanoparticles and applied for biodiesel production. The results indicated more affinity of the ROL toward its substrate upon immobilization, as revealed by a lower Km value for the immobilized ROL compared to its free counterpart. Intrinsic fluorescence spectroscopy indicated a lower intensity for ROL immobilized on CoFe2O4 nanoparticles. Besides, immobilized ROL steady state anisotropy measurements presented lower values, which implied assembly of ROL molecules on magnetic nanoparticles upon immobilization as well as their restricted rotation upon covalent attachment. Thermal stability analysis revealed improved activity at higher temperatures for the immobilized enzyme compared to its free counterpart. Accordingly, Pace analysis to determine protein thermal stability revealed preservation of the protein conformation in the presence of increasing temperatures upon immobilization on nanoparticles. Finally, ROL immobilized on CoFe2O4 nanoparticles exhibited improved efficiency of biodiesel production in agreement with thermal activity profile. Therefore, the authors suggest application of the lipase molecules immobilized on CoFe2O4 nanoparticles for more efficient biodiesel production.
ISSN:1004-9541
2210-321X
DOI:10.1016/j.cjche.2020.11.003