A Preliminary Study on a Specifically Expressed Arabidopsis Promotor in Vascular Bundle

O4; From a population of about 3500 single plants in Arabidopsis promoter trapping bank, one plant whose GUS-gene had been specifically expressed in vascular bundle, was screened by the method of gus tissue staining. The T-DNA flanking sequence was amplified using TAILPCR. This band will be purified...

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Bibliographic Details
Published in:Plasma science & technology 2003-04, Vol.5 (2), p.1755-1760
Main Authors: Gu, Yun-hong, Xie, Chuan-xiao, Wu, Li-fang, Yu, Zeng-liang, Qin, Guang-yong, Huo, Yu-ping
Format: Article
Language:English
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Summary:O4; From a population of about 3500 single plants in Arabidopsis promoter trapping bank, one plant whose GUS-gene had been specifically expressed in vascular bundle, was screened by the method of gus tissue staining. The T-DNA flanking sequence was amplified using TAILPCR. This band will be purified and connected to TA cloning vector. After sequencing and searching in the genebank, its function will be demonatrated through transformation.
ISSN:1009-0630
DOI:10.1088/1009-0630/5/2/012