新型比色荧光双通道探针用于硫化氢的检测

以7-硝基苯并呋咱(NBD)为荧光团,经一步有机合成,高产率制备比色荧光双通道探针,用于检测环境和体内硫化氢(H2 S)的含量.以无水Na2 S为H2 S供体,考察了探针的选择性、灵敏性等性能.结果表明,探针荧光强度与H2 S浓度在5～50μmol/L浓度范围内呈良好的线性关系,检出限为0.125μmol/L,同时反应溶液由无色变为紫色.采用MTT法测试探针的细胞毒性,结果表明,在探针浓度低于50μmol/L条件下,细胞存活率高于90％.激光扫描共聚焦显微成像结果表明,探针可靶向细胞溶酶体,可对溶酶体内H2 S进行成像研究....

Full description

Saved in:
Bibliographic Details
Published in:分析化学 2019, Vol.47 (12), p.1915-1921
Main Authors: 王肖莉, 姚猛, 李引, 魏超, 王美
Format: Article
Language:Chinese
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
cited_by
cites
container_end_page 1921
container_issue 12
container_start_page 1915
container_title 分析化学
container_volume 47
creator 王肖莉
姚猛
李引
魏超
王美
description 以7-硝基苯并呋咱(NBD)为荧光团,经一步有机合成,高产率制备比色荧光双通道探针,用于检测环境和体内硫化氢(H2 S)的含量.以无水Na2 S为H2 S供体,考察了探针的选择性、灵敏性等性能.结果表明,探针荧光强度与H2 S浓度在5～50μmol/L浓度范围内呈良好的线性关系,检出限为0.125μmol/L,同时反应溶液由无色变为紫色.采用MTT法测试探针的细胞毒性,结果表明,在探针浓度低于50μmol/L条件下,细胞存活率高于90％.激光扫描共聚焦显微成像结果表明,探针可靶向细胞溶酶体,可对溶酶体内H2 S进行成像研究.
doi_str_mv 10.19756/j.issn.0253-3820.191451
format article
fullrecord <record><control><sourceid>wanfang_jour</sourceid><recordid>TN_cdi_wanfang_journals_fxhx201912008</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><wanfj_id>fxhx201912008</wanfj_id><sourcerecordid>fxhx201912008</sourcerecordid><originalsourceid>FETCH-wanfang_journals_fxhx2019120083</originalsourceid><addsrcrecordid>eNpjYFAwNNAztDQ3NdPP0sssLs7TMzAyNdY1tjACCRuamBqyMHDChTgYeIuLM5MMDAwNDUwsTC04GSyfTdvwdF73s_VTXnRuetG7_Glr59P-npcNs142Tn7Wt-jlpI7nU1Y82dX3fOHqpz3Tnm1Y9HxWy7PFDc-2dvMwsKYl5hSn8kJpbgZVN9cQZw_d8sS8tMS89Pis_NKiPKBMfFpFRoWRAdA1RgYGFsbEqgMAcrBSLw</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype></control><display><type>article</type><title>新型比色荧光双通道探针用于硫化氢的检测</title><source>Elsevier</source><creator>王肖莉 ; 姚猛 ; 李引 ; 魏超 ; 王美</creator><creatorcontrib>王肖莉 ; 姚猛 ; 李引 ; 魏超 ; 王美</creatorcontrib><description>以7-硝基苯并呋咱(NBD)为荧光团,经一步有机合成,高产率制备比色荧光双通道探针,用于检测环境和体内硫化氢(H2 S)的含量.以无水Na2 S为H2 S供体,考察了探针的选择性、灵敏性等性能.结果表明,探针荧光强度与H2 S浓度在5～50μmol/L浓度范围内呈良好的线性关系,检出限为0.125μmol/L,同时反应溶液由无色变为紫色.采用MTT法测试探针的细胞毒性,结果表明,在探针浓度低于50μmol/L条件下,细胞存活率高于90％.激光扫描共聚焦显微成像结果表明,探针可靶向细胞溶酶体,可对溶酶体内H2 S进行成像研究.</description><identifier>ISSN: 0253-3820</identifier><identifier>DOI: 10.19756/j.issn.0253-3820.191451</identifier><language>chi</language><publisher>河北大学化学与环境科学学院,保定,071002%河北大学化学与环境科学学院,保定071002</publisher><ispartof>分析化学, 2019, Vol.47 (12), p.1915-1921</ispartof><rights>Copyright © Wanfang Data Co. Ltd. All Rights Reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://www.wanfangdata.com.cn/images/PeriodicalImages/fxhx/fxhx.jpg</thumbnail><link.rule.ids>314,780,784,4024,27923,27924,27925</link.rule.ids></links><search><creatorcontrib>王肖莉</creatorcontrib><creatorcontrib>姚猛</creatorcontrib><creatorcontrib>李引</creatorcontrib><creatorcontrib>魏超</creatorcontrib><creatorcontrib>王美</creatorcontrib><title>新型比色荧光双通道探针用于硫化氢的检测</title><title>分析化学</title><description>以7-硝基苯并呋咱(NBD)为荧光团,经一步有机合成,高产率制备比色荧光双通道探针,用于检测环境和体内硫化氢(H2 S)的含量.以无水Na2 S为H2 S供体,考察了探针的选择性、灵敏性等性能.结果表明,探针荧光强度与H2 S浓度在5～50μmol/L浓度范围内呈良好的线性关系,检出限为0.125μmol/L,同时反应溶液由无色变为紫色.采用MTT法测试探针的细胞毒性,结果表明,在探针浓度低于50μmol/L条件下,细胞存活率高于90％.激光扫描共聚焦显微成像结果表明,探针可靶向细胞溶酶体,可对溶酶体内H2 S进行成像研究.</description><issn>0253-3820</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><recordid>eNpjYFAwNNAztDQ3NdPP0sssLs7TMzAyNdY1tjACCRuamBqyMHDChTgYeIuLM5MMDAwNDUwsTC04GSyfTdvwdF73s_VTXnRuetG7_Glr59P-npcNs142Tn7Wt-jlpI7nU1Y82dX3fOHqpz3Tnm1Y9HxWy7PFDc-2dvMwsKYl5hSn8kJpbgZVN9cQZw_d8sS8tMS89Pis_NKiPKBMfFpFRoWRAdA1RgYGFsbEqgMAcrBSLw</recordid><startdate>2019</startdate><enddate>2019</enddate><creator>王肖莉</creator><creator>姚猛</creator><creator>李引</creator><creator>魏超</creator><creator>王美</creator><general>河北大学化学与环境科学学院,保定,071002%河北大学化学与环境科学学院,保定071002</general><general>河北省阻燃材料与加工技术工程技术研究中心,保定071002</general><scope>2B.</scope><scope>4A8</scope><scope>92I</scope><scope>93N</scope><scope>PSX</scope><scope>TCJ</scope></search><sort><creationdate>2019</creationdate><title>新型比色荧光双通道探针用于硫化氢的检测</title><author>王肖莉 ; 姚猛 ; 李引 ; 魏超 ; 王美</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-wanfang_journals_fxhx2019120083</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>chi</language><creationdate>2019</creationdate><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>王肖莉</creatorcontrib><creatorcontrib>姚猛</creatorcontrib><creatorcontrib>李引</creatorcontrib><creatorcontrib>魏超</creatorcontrib><creatorcontrib>王美</creatorcontrib><collection>Wanfang Data Journals - Hong Kong</collection><collection>WANFANG Data Centre</collection><collection>Wanfang Data Journals</collection><collection>万方数据期刊 - 香港版</collection><collection>China Online Journals (COJ)</collection><collection>China Online Journals (COJ)</collection><jtitle>分析化学</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>王肖莉</au><au>姚猛</au><au>李引</au><au>魏超</au><au>王美</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>新型比色荧光双通道探针用于硫化氢的检测</atitle><jtitle>分析化学</jtitle><date>2019</date><risdate>2019</risdate><volume>47</volume><issue>12</issue><spage>1915</spage><epage>1921</epage><pages>1915-1921</pages><issn>0253-3820</issn><abstract>以7-硝基苯并呋咱(NBD)为荧光团,经一步有机合成,高产率制备比色荧光双通道探针,用于检测环境和体内硫化氢(H2 S)的含量.以无水Na2 S为H2 S供体,考察了探针的选择性、灵敏性等性能.结果表明,探针荧光强度与H2 S浓度在5～50μmol/L浓度范围内呈良好的线性关系,检出限为0.125μmol/L,同时反应溶液由无色变为紫色.采用MTT法测试探针的细胞毒性,结果表明,在探针浓度低于50μmol/L条件下,细胞存活率高于90％.激光扫描共聚焦显微成像结果表明,探针可靶向细胞溶酶体,可对溶酶体内H2 S进行成像研究.</abstract><pub>河北大学化学与环境科学学院,保定,071002%河北大学化学与环境科学学院,保定071002</pub><doi>10.19756/j.issn.0253-3820.191451</doi></addata></record>
fulltext fulltext
identifier ISSN: 0253-3820
ispartof 分析化学, 2019, Vol.47 (12), p.1915-1921
issn 0253-3820
language chi
recordid cdi_wanfang_journals_fxhx201912008
source Elsevier
title 新型比色荧光双通道探针用于硫化氢的检测
url http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-05T00%3A55%3A55IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-wanfang_jour&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=%E6%96%B0%E5%9E%8B%E6%AF%94%E8%89%B2%E8%8D%A7%E5%85%89%E5%8F%8C%E9%80%9A%E9%81%93%E6%8E%A2%E9%92%88%E7%94%A8%E4%BA%8E%E7%A1%AB%E5%8C%96%E6%B0%A2%E7%9A%84%E6%A3%80%E6%B5%8B&rft.jtitle=%E5%88%86%E6%9E%90%E5%8C%96%E5%AD%A6&rft.au=%E7%8E%8B%E8%82%96%E8%8E%89&rft.date=2019&rft.volume=47&rft.issue=12&rft.spage=1915&rft.epage=1921&rft.pages=1915-1921&rft.issn=0253-3820&rft_id=info:doi/10.19756/j.issn.0253-3820.191451&rft_dat=%3Cwanfang_jour%3Efxhx201912008%3C/wanfang_jour%3E%3Cgrp_id%3Ecdi_FETCH-wanfang_journals_fxhx2019120083%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_id=info:pmid/&rft_wanfj_id=fxhx201912008&rfr_iscdi=true