Clinical Application of Fluorescence in Situ Hybridization (FISH) to Detect HER-2 Gene in Breast Cancer

Objective: To investigate the clinical application of the detection of HER-2 gene by lfuorescence in situ hybridization (FISH) in breast cancer and the correlation between HER-2 gene ampliifcation and clinicopathology of breast cancer. Methods:Parafifn-embedded breast inifltrating ductal carcinoma f...

Full description

Saved in:
Bibliographic Details
Published in:国际转化医学杂志（英文版） 2014-06 (2), p.326-330
Main Authors: Maurie Buehler, Ellie Guardino, Jung Sik Park, Eun Jeong Jang
Format: Article
Language:English
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
cited_by
cites
container_end_page 330
container_issue 2
container_start_page 326
container_title 国际转化医学杂志（英文版）
container_volume
creator Maurie Buehler
Ellie Guardino
Jung Sik Park
Eun Jeong Jang
description Objective: To investigate the clinical application of the detection of HER-2 gene by lfuorescence in situ hybridization (FISH) in breast cancer and the correlation between HER-2 gene ampliifcation and clinicopathology of breast cancer. Methods:Parafifn-embedded breast inifltrating ductal carcinoma from 48 patients were detected by FISH and immunohistochemistry (IHC) respectively for comparing the results of two methods. Results: HER-2 protein expressions were classified into three groups (3+/2+/1+ or 0) and the positive rates of HER-2 gene ampliifcation by FISH were 77.8%, 57.1% and 10.5%, respectively. Of the 29 cases with positive axillary lymph node, 12 were with HER-2 gene ampliifcation (P0.05). Conclusion:The false positive and negative rates are higher in HER-2 protein expression by IHC. Compared with IHC, FISH, being more effective and precise, can be applied extensively in clinic. HER-2 gene ampliifcation is concerned with axillary nodes metastases.
format article
fullrecord <record><control><sourceid>wanfang_jour</sourceid><recordid>TN_cdi_wanfang_journals_gjzhyxzz_e201402010</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><wanfj_id>gjzhyxzz_e201402010</wanfj_id><sourcerecordid>gjzhyxzz_e201402010</sourcerecordid><originalsourceid>FETCH-LOGICAL-w121t-5448197b66df4e3f6299d7e753c69f1e76526d1cdee51c539571a096ea3031e83</originalsourceid><addsrcrecordid>eNpNjc9LwzAcxYMoOOb-hxwVKeRHkzTHWdd1MBCcnkuWfltTSjralLn-9Rbnwct77_De592gBWNMRZLr-PZfvkerYWgIIVQkUiu5QHXaOu-safH6dGrnEFzncVfhrB27HgYL3gJ2Hh9cGHF-OfaudNO19ZjtDvkTDh1-hQA24HzzHjG8Bf-7eOnBDAGnZib0D-iuMu0Aqz9fos9s85Hm0f5tu0vX--hMGQ2RiOOEanWUsqxi4JVkWpcKlOBW6oqCkoLJktoSQFAruBaKGqIlGE44hYQv0fOVeza-Mr4umm7s_fxY1M30dfmepgIYoTGZhfAfYOFVtQ</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype></control><display><type>article</type><title>Clinical Application of Fluorescence in Situ Hybridization (FISH) to Detect HER-2 Gene in Breast Cancer</title><source>IngentaConnect Journals</source><creator>Maurie Buehler ; Ellie Guardino ; Jung Sik Park ; Eun Jeong Jang</creator><creatorcontrib>Maurie Buehler ; Ellie Guardino ; Jung Sik Park ; Eun Jeong Jang</creatorcontrib><description>Objective: To investigate the clinical application of the detection of HER-2 gene by lfuorescence in situ hybridization (FISH) in breast cancer and the correlation between HER-2 gene ampliifcation and clinicopathology of breast cancer. Methods:Parafifn-embedded breast inifltrating ductal carcinoma from 48 patients were detected by FISH and immunohistochemistry (IHC) respectively for comparing the results of two methods. Results: HER-2 protein expressions were classified into three groups (3+/2+/1+ or 0) and the positive rates of HER-2 gene ampliifcation by FISH were 77.8%, 57.1% and 10.5%, respectively. Of the 29 cases with positive axillary lymph node, 12 were with HER-2 gene ampliifcation (P&lt;0.05). Of the 10 cases with negative estrogen receptor (ER) and progesterone receptor (PR), 7 were with HER-2 gene ampliifcation (P&gt;0.05). Conclusion:The false positive and negative rates are higher in HER-2 protein expression by IHC. Compared with IHC, FISH, being more effective and precise, can be applied extensively in clinic. HER-2 gene ampliifcation is concerned with axillary nodes metastases.</description><identifier>ISSN: 2227-6394</identifier><identifier>EISSN: 2227-6394</identifier><language>eng</language><publisher>Department of Medical 0ncology, University of Nebraska Medical Center, Nebraska, USA%Department of 0ncology, Kyungpook National University Hospital, Kyungpook National University School of Medicine, Daegu, Korea%Department of Thoracic Surgery, Korea University Anam Hospital, Seongbuk-gu, Seoul, Korea</publisher><ispartof>国际转化医学杂志（英文版）, 2014-06 (2), p.326-330</ispartof><rights>Copyright © Wanfang Data Co. Ltd. All Rights Reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://www.wanfangdata.com.cn/images/PeriodicalImages/gjzhyxzz-e/gjzhyxzz-e.jpg</thumbnail><link.rule.ids>314,780,784</link.rule.ids></links><search><creatorcontrib>Maurie Buehler</creatorcontrib><creatorcontrib>Ellie Guardino</creatorcontrib><creatorcontrib>Jung Sik Park</creatorcontrib><creatorcontrib>Eun Jeong Jang</creatorcontrib><title>Clinical Application of Fluorescence in Situ Hybridization (FISH) to Detect HER-2 Gene in Breast Cancer</title><title>国际转化医学杂志（英文版）</title><description>Objective: To investigate the clinical application of the detection of HER-2 gene by lfuorescence in situ hybridization (FISH) in breast cancer and the correlation between HER-2 gene ampliifcation and clinicopathology of breast cancer. Methods:Parafifn-embedded breast inifltrating ductal carcinoma from 48 patients were detected by FISH and immunohistochemistry (IHC) respectively for comparing the results of two methods. Results: HER-2 protein expressions were classified into three groups (3+/2+/1+ or 0) and the positive rates of HER-2 gene ampliifcation by FISH were 77.8%, 57.1% and 10.5%, respectively. Of the 29 cases with positive axillary lymph node, 12 were with HER-2 gene ampliifcation (P&lt;0.05). Of the 10 cases with negative estrogen receptor (ER) and progesterone receptor (PR), 7 were with HER-2 gene ampliifcation (P&gt;0.05). Conclusion:The false positive and negative rates are higher in HER-2 protein expression by IHC. Compared with IHC, FISH, being more effective and precise, can be applied extensively in clinic. HER-2 gene ampliifcation is concerned with axillary nodes metastases.</description><issn>2227-6394</issn><issn>2227-6394</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><recordid>eNpNjc9LwzAcxYMoOOb-hxwVKeRHkzTHWdd1MBCcnkuWfltTSjralLn-9Rbnwct77_De592gBWNMRZLr-PZfvkerYWgIIVQkUiu5QHXaOu-safH6dGrnEFzncVfhrB27HgYL3gJ2Hh9cGHF-OfaudNO19ZjtDvkTDh1-hQA24HzzHjG8Bf-7eOnBDAGnZib0D-iuMu0Aqz9fos9s85Hm0f5tu0vX--hMGQ2RiOOEanWUsqxi4JVkWpcKlOBW6oqCkoLJktoSQFAruBaKGqIlGE44hYQv0fOVeza-Mr4umm7s_fxY1M30dfmepgIYoTGZhfAfYOFVtQ</recordid><startdate>20140630</startdate><enddate>20140630</enddate><creator>Maurie Buehler</creator><creator>Ellie Guardino</creator><creator>Jung Sik Park</creator><creator>Eun Jeong Jang</creator><general>Department of Medical 0ncology, University of Nebraska Medical Center, Nebraska, USA%Department of 0ncology, Kyungpook National University Hospital, Kyungpook National University School of Medicine, Daegu, Korea%Department of Thoracic Surgery, Korea University Anam Hospital, Seongbuk-gu, Seoul, Korea</general><scope>2B.</scope><scope>4A8</scope><scope>92I</scope><scope>93N</scope><scope>PSX</scope><scope>TCJ</scope></search><sort><creationdate>20140630</creationdate><title>Clinical Application of Fluorescence in Situ Hybridization (FISH) to Detect HER-2 Gene in Breast Cancer</title><author>Maurie Buehler ; Ellie Guardino ; Jung Sik Park ; Eun Jeong Jang</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-w121t-5448197b66df4e3f6299d7e753c69f1e76526d1cdee51c539571a096ea3031e83</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Maurie Buehler</creatorcontrib><creatorcontrib>Ellie Guardino</creatorcontrib><creatorcontrib>Jung Sik Park</creatorcontrib><creatorcontrib>Eun Jeong Jang</creatorcontrib><collection>Wanfang Data Journals - Hong Kong</collection><collection>WANFANG Data Centre</collection><collection>Wanfang Data Journals</collection><collection>万方数据期刊 - 香港版</collection><collection>China Online Journals (COJ)</collection><collection>China Online Journals (COJ)</collection><jtitle>国际转化医学杂志（英文版）</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Maurie Buehler</au><au>Ellie Guardino</au><au>Jung Sik Park</au><au>Eun Jeong Jang</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Clinical Application of Fluorescence in Situ Hybridization (FISH) to Detect HER-2 Gene in Breast Cancer</atitle><jtitle>国际转化医学杂志（英文版）</jtitle><date>2014-06-30</date><risdate>2014</risdate><issue>2</issue><spage>326</spage><epage>330</epage><pages>326-330</pages><issn>2227-6394</issn><eissn>2227-6394</eissn><abstract>Objective: To investigate the clinical application of the detection of HER-2 gene by lfuorescence in situ hybridization (FISH) in breast cancer and the correlation between HER-2 gene ampliifcation and clinicopathology of breast cancer. Methods:Parafifn-embedded breast inifltrating ductal carcinoma from 48 patients were detected by FISH and immunohistochemistry (IHC) respectively for comparing the results of two methods. Results: HER-2 protein expressions were classified into three groups (3+/2+/1+ or 0) and the positive rates of HER-2 gene ampliifcation by FISH were 77.8%, 57.1% and 10.5%, respectively. Of the 29 cases with positive axillary lymph node, 12 were with HER-2 gene ampliifcation (P&lt;0.05). Of the 10 cases with negative estrogen receptor (ER) and progesterone receptor (PR), 7 were with HER-2 gene ampliifcation (P&gt;0.05). Conclusion:The false positive and negative rates are higher in HER-2 protein expression by IHC. Compared with IHC, FISH, being more effective and precise, can be applied extensively in clinic. HER-2 gene ampliifcation is concerned with axillary nodes metastases.</abstract><pub>Department of Medical 0ncology, University of Nebraska Medical Center, Nebraska, USA%Department of 0ncology, Kyungpook National University Hospital, Kyungpook National University School of Medicine, Daegu, Korea%Department of Thoracic Surgery, Korea University Anam Hospital, Seongbuk-gu, Seoul, Korea</pub><tpages>5</tpages></addata></record>
fulltext fulltext
identifier ISSN: 2227-6394
ispartof 国际转化医学杂志（英文版）, 2014-06 (2), p.326-330
issn 2227-6394
2227-6394
language eng
recordid cdi_wanfang_journals_gjzhyxzz_e201402010
source IngentaConnect Journals
title Clinical Application of Fluorescence in Situ Hybridization (FISH) to Detect HER-2 Gene in Breast Cancer
url http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-06T07%3A59%3A44IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-wanfang_jour&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Clinical%20Application%20of%20Fluorescence%20in%20Situ%20Hybridization%20(FISH)%20to%20Detect%20HER-2%20Gene%20in%20Breast%20Cancer&rft.jtitle=%E5%9B%BD%E9%99%85%E8%BD%AC%E5%8C%96%E5%8C%BB%E5%AD%A6%E6%9D%82%E5%BF%97%EF%BC%88%E8%8B%B1%E6%96%87%E7%89%88%EF%BC%89&rft.au=Maurie%20Buehler&rft.date=2014-06-30&rft.issue=2&rft.spage=326&rft.epage=330&rft.pages=326-330&rft.issn=2227-6394&rft.eissn=2227-6394&rft_id=info:doi/&rft_dat=%3Cwanfang_jour%3Egjzhyxzz_e201402010%3C/wanfang_jour%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-w121t-5448197b66df4e3f6299d7e753c69f1e76526d1cdee51c539571a096ea3031e83%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_id=info:pmid/&rft_wanfj_id=gjzhyxzz_e201402010&rfr_iscdi=true