Effect of hTERT Gene Expression on Proliferation and Apoptosis of Cervical Cancer Caski Cells by Lentivirus-Mediated Small Interfering RNA

Objective: To explore the feasibility of lentivirus-mediated small interfering RNA (siRNA) silencing the human telomerase reverse transcriptase (hTERT) and its inlfuence on the proliferation and apoptosis of cervical cancer Caski cells. Methods: The cervical cancer Caski cells were transfected by le...

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Published in:国际转化医学杂志（英文版） 2016-06, Vol.4 (2), p.114-120
Main Authors: MO Ling-zhao, LI De-quan, XIA Yu, CHEN Chang-xian, PANG Xiao-fen, LING Dan
Format: Article
Language:English
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Summary:Objective: To explore the feasibility of lentivirus-mediated small interfering RNA (siRNA) silencing the human telomerase reverse transcriptase (hTERT) and its inlfuence on the proliferation and apoptosis of cervical cancer Caski cells. Methods: The cervical cancer Caski cells were transfected by lentivirus expression vectors anti-hTERT-TV and NC-LV which had been constructed in advance. There were three groups in the experiment, namely blank group (NC group), negative control group (NC-LV group) and interference group (anti-hTERT-LV group). The expression level of hTERT mRNA was determined by real-time lfuorescent quantitative polymerase chain reaction (PCR), while the cell cycle and apoptosis were detected by lfow cytometry. Results: The relative expression level of hTERT mRNA in anti-hTERT-LV group was signiifcantly lower than those in NC group and NC-LV group (P
ISSN:2227-6394
2227-6394
DOI:10.11910/2227-6394.2016.04.02.07