Genetic diversity analysis of spawner and recaptured populations of Chinese shrimp (Fenneropenaeus chinensis) during stock enhancement in the Bohai Bay based on an SSR marker

• Published in: Acta oceanologica Sinica 2016-08, Vol.35 (8), p.51-56
• Main Authors: Wang, Mosang, Wang, Weiji, Xiao, Guangxia, Liu, Kefeng, Hu, Yulong, Tian, Tao, Kong, Jie, Jin, Xianshi
• Format: Article
• Language: English
• Subjects: Alleles; Brackish; Climatology; Coefficients; Decapoda; Earth and Environmental Science; Earth Sciences; Ecology; Economic impact; Engineering Fluid Dynamics; Environmental Chemistry; Environmental conditions; Evolution; Fenneropenaeus chinensis; Fishing; Fishing effort; Genetic analysis; Genetic diversity; Genetic markers; Genetic variation; Heterozygosity; Inbreeding; Inbreeding depression; Low level; Marine; Marine & Freshwater Sciences; Marine crustaceans; Microsatellites; Oceanography; Polymorphism; Population genetics; Population number; Populations; Restocking; Shrimps; Spawning populations
• ISSN: 0253-505X; 1869-1099
• DOI: 10.1007/s13131-016-0830-0

Eight microsatellite markers were used to analyze genetic diversity, level of inbreeding, and effective population size of spawner and recaptured populations of Chinese shrimp ( Fenneropenaeus chinensis ) during stock enhancement in the Bohai Bay in 2013. A total of 254 and 238 alleles were identified in the spawner and recaptured populations, respectively, and the numbers of alleles ( N a ) were 8–63 and 6–60, respectively. The numbers of effective alleles ( N e ) were 2.52–21.60 and 2.67–20.72, respectively. The polymorphism information content ranged from 0.529 to 0.952. The observed heterozygosity ( H o ) values (0.638–0.910 and 0.712–0.927) were lower than the expected heterozygosity ( H e ) values (0.603–0.954 and 0.625–0.952), which indicated that the two populations possessed a rich genetic diversity. In 16 tests (2 populations×8 loci), 13 tests deviated from the Hardy-Weinberg equilibrium. F is values were positive at seven loci and the inbreeding coefficients ( F ) of the two populations estimated by trioML were 13.234% and 11.603%, suggesting that there was a relatively high degree of inbreeding. A certain level of inbreeding depression had occurred in the Chinese shrimp population. F st values ranged from 0 to 0.059, with a mean of 0.028, displaying a low level of genetic differentiation in the two populations. Effective population sizes (3 060.2 and 3 842.8) were higher than the minimum number suggested for retaining the evolutionary potential to adapt to new environmental conditions. For enhancement activity in 2014, the ideal number of captured shrimp spawners should have ranged from 7 686 to 19 214 to maintain genetic diversity and effective population size. Further strategies to adjust the balance of economic cost, fishing effort and ideal number of shrimp spawners to maintain a satisfactory effective population size for ensuring the sustainability of Chinese shrimp are proposed.