Biomass, nutrient uptake and fatty acid composition of Chlamydomonas sp. ICE-L in response to different nitrogen sources

• Published in: Acta oceanologica Sinica 2017-02, Vol.36 (2), p.105-110
• Main Authors: An, Meiling, Wang, Yibin, Liu, Fangming, Qi, Xiaoqing, Zheng, Zhou, Ye, Naihao, Sun, Chengjun, Miao, Jinlai
• Format: Article
• Language: English
• Subjects: Algae; Algal growth; Ammonium chloride; Ammonium nitrate; Biodiesel fuels; Biofuels; Biomass; Chlamydomonas; Climatology; Earth and Environmental Science; Earth Sciences; Ecology; Engineering Fluid Dynamics; Environmental Chemistry; Fatty acid composition; Fatty acids; Growth rate; Lipids; Marine; Marine & Freshwater Sciences; Media; Nitrogen; Nitrogen removal; Nitrogen sources; Nutrient uptake; Oceanography; Phytoplankton; Polyunsaturated fatty acids; 养分吸收; 南极; 多不饱和脂肪酸; 氮源; 燃料生产; 生物量生产; 脂肪酸组成; 衣藻
• ISSN: 0253-505X; 1869-1099
• DOI: 10.1007/s13131-017-0984-4

Nitrogen removal from media by microalgae provides the potential benefit of producing lipids for biodiesel and biomass. However, research is limited on algal growth and biomass under different nitrogen sources and provides little insight in terms of biofuel production. We studied the influences of nitrogen sources on cell growth and lipid accumulation of Chlamydomonas sp. ICE-L, one of a promising oil rich micro algal species. Chlamydomonas sp.ICE-L grown in NH_4 Cl medium had maximum growth rate. While the highest dry biomass of 0.28 g/L was obtained in media containing NH_4NO_3, the highest lipid content of 0.21 g/g was achieved under nitrogendeficiency condition with a dry biomass of 0.24 g/L. In terms of total polyunsaturated fatty acids（PUFAs）production, NH_4NO_3 and NH_4 Cl media performed better than nitrogen-deficiency and KNO_3 media.Furthermore, NH_4NO_3 and NH_4 Cl media elucidated better results on C18：3 and C20：5 productions while KNO_3and-N conditions were better in C16：0, C18：1 and C18：2, comparatively.