Identification and characterization of a delta-12 fatty acid desaturase gene from marine microalgae Isochrysis galbana

The cDNA of the delta-12 fatty acid desaturase gene, IgFAD2 , was cloned from the marine microalgae Isochrysis galbana , a species capable of producing docosahexaenoic acid. Sequence analysis indicated that the open reading frame measured a length of 1 158 bp and encoded 386 amino acids with a predi...

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Bibliographic Details
Published in:Acta oceanologica Sinica 2019-02, Vol.38 (2), p.107-113
Main Authors: Han, Xiaotian, Wang, Shuai, Zheng, Li, Liu, Wanshun
Format: Article
Language:English
Subjects:
Algae
Amino acid sequence
Amino acids
Aquatic microorganisms
Climatology
Complementary DNA
Computer applications
Desaturase
DNA
Docosahexaenoic acid
Earth and Environmental Science
Earth Sciences
Ecology
Engineering Fluid Dynamics
Environmental Chemistry
Environmental stress
Fatty acids
Histidine
Isochrysis galbana
Linoleic acid
Low temperature
Marine & Freshwater Sciences
Membranes
Microalgae
Molecular weight
Nucleotide sequence
Oceanography
Oleic acid
PCR
Phytoplankton
Plasmids
Protein sequencing
Salinity
Salinity effects
Sequence analysis
Sequencing
Starvation
Transcription
Yeast
Yeasts
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Summary:The cDNA of the delta-12 fatty acid desaturase gene, IgFAD2 , was cloned from the marine microalgae Isochrysis galbana , a species capable of producing docosahexaenoic acid. Sequence analysis indicated that the open reading frame measured a length of 1 158 bp and encoded 386 amino acids with a predicted molecular weight of 42.8 kDa and an isoelectric point of 9.2. Computational analysis of the protein sequence of IgFAD2 showed typical features of membrane-bound desaturase such as three conserved histidine boxes along with four membrane-spanning regions that were universally present among plant desaturases. Quantitative real-time PCR results showed that the abundance of IgFAD2 transcript was significantly upregulated under different environmental stresses including low temperature (15°C), high salinity (salinity of 62 and 93), and nitrogen starvation (220 μmol/L). Heterologous expression indicated that yeast cells transformed with a plasmid construct containing IgFAD2 could convert endogenous oleic acid (18:1 Δ9 , OA) into linoleic acid (18:2 Δ9 , 12, LA). These findings confirm that I. galbana IgFAD2 plays important roles in the biosynthetic pathways of unsaturated fatty acids.
ISSN:0253-505X
1869-1099
DOI:10.1007/s13131-019-1354-1