mRNA expression of CYP4 in marine polychaete Marphysa sanguinea in response to benzo[a]pyrene

• Published in: Acta oceanologica Sinica 2019-06, Vol.38 (6), p.46-53
• Main Authors: Li, Wanjuan, Zhao, Huan, Ba, Fuyang, Li, Shaojuan, Sun, Xiupeng, Yang, Dazuo, Zhou, Yibing
• Format: Article
• Language: English
• Subjects: Amino acid sequence; Amino acid sequences; Amino acids; Aromatic hydrocarbons; Benzo(a)pyrene; Climatology; Complementary DNA; CYP4 gene; DNA; Earth and Environmental Science; Earth Sciences; Ecology; Engineering Fluid Dynamics; Environmental Chemistry; Gene expression; Homology; Marine & Freshwater Sciences; Marphysa sanguinea; Metabolism; Nucleotide sequence; Oceanography; PCR; Polymerase chain reaction; Pyrene; Toxicity
• ISSN: 0253-505X; 1869-1099
• DOI: 10.1007/s13131-019-1362-1

Rapid amplification of cDNA ends (RACE) and real-time polymerase chain reaction (RT-PCR) were carried out to analyze the CYP4 gene expression in polychaete Marphysa sanguinea exposed to benzo[a]pyrene (BaP) in this study. The full length of MsCYP4 cDNA was 2 470 bp, and it encoded 512 amino acids. The deduced amino acid sequence showed 47% identity with CYP4F from frog Xenopus tropicalis and shared high homology with other known CYP4 sequences. To analyse the role of CYP4 in protecting M. sanguinea from BaP exposure, three BaP groups were established: 0.5, 5 and 50 μg/L. Polychaetes were sampled after 3, 7 and 12 d. At 0.5 μg/L, the effect of BaP on MsCYP4 gene expression increased with time prolonged. MsCYP4 gene expression curve showed U-shaped trend with time in 5 and 50 μg/L BaP groups. Therefore, MsCYP4 gene may play an important role in maintaining the balance of cellular metabolism and protecting M. sanguinea from BaP toxicity.