Effect of seed sludge on characteristics and microbial community of aerobic granular sludge

• Published in: Journal of environmental sciences (China) 2010-09, Vol.22 (9), p.1312-1318
• Main Authors: Song, Zhiwei, Pan, Yuejun, Zhang, Kun, Ren, Nanqi, Wang, Aijie
• Format: Article
• Language: English
• Subjects: aerobic granular sludge; Aerobiosis; Denaturing Gradient Gel Electrophoresis; Devosia; Granular materials; Granules; Inoculum; Lactococcus; microbial community; Microorganisms; Microscopy, Electron, Scanning; Paracoccus; PCR-DGGE; Polymerase Chain Reaction; Pseudomonas; Scanning electron microscopy; seed sludge; Seeds; Sequencing; Sewage - microbiology; Sludge; Waste Disposal, Fluid; 凝胶电泳技术; 城镇污水处理厂; 好氧颗粒污泥; 序批式反应器; 微生物群落结构; 扫描电子显微镜; 接种污泥
• ISSN: 1001-0742; 1878-7320
• DOI: 10.1016/s1001-0742(09)60256-4

Aerobic granular sludge was cultivated by using different kinds of seed sludge in sequencing batch airlift reactor. The influence of seed sludge on physical and chemical properties of granular sludge was studied; the microbial community structure was probed by using scanning electron microscope and polymerase chain reaction-denaturing gradient gel electrophoresis （PCR-DGGE）. The results showed that seed sludge played an important role on the formation of aerobic granules. Seed sludge taken from beer wastewater treatment plant （inoculum A） was more suitable for cultivating aerobic granules than that of sludge from municipal wastewater treatment plant （inoculurn B）. Cultivated with inoculum A, large amount of mature granules formed after 35 days operation, its SVI reached 32.75 mL/g, and SOUR of granular sludge was beyond 1.10 mg/（g.min）. By contrast, it needed 56 days obtaining mature granules using inoculum B. DGGE profiles indicated that the dominant microbial species in mature granules were 18 and 11 OTU when inoculum A and B were respectively employed as seed sludge. The sequencing results suggested that dominant species in mature granules cultivated by inoculum A were Paracoccus sp., Devosia hwasunensi, Pseudoxanthomonas sp., while the dominant species were Lactococcus raffinolactis and Pseudomonas sp. in granules developed from inoculum B.