Biodegradation of beet molasses vinasse by a mixed culture of micro organisms：Effect of aeration conditions and pH control

• Published in: Journal of environmental sciences (China) 2011-11, Vol.23 (11), p.1823-1830
• Main Authors: Lutoslawski, Krzysztof, Ryznar-Luty, Agnieszka, Cibis, Edmund, Krzywonos, Malgorzata, Miskiewicz, Tadeusz
• Format: Article
• Language: English
• Subjects: Aeration; aerobic biodégradation; Bacillus; batch process; beet molasses vinasse; Beta vulgaris - metabolism; Betaines; Bifidobacterium; Biodegradation; Biodegradation, Environmental; Biofuels; Bioreactors - microbiology; Glycerol - isolation & purification; Hydrogen-Ion Concentration; Industrial Microbiology - methods; Industrial Waste; Lactobacillus; Lactococcus; mesophilic conditions; Microorganisms; mixed culture of microorganisms; Molasses; Nitrogen - isolation & purification; Oxygen demand; Phosphorus - isolation & purification; pH值控制; Reduction; Rhodopseudomonas; Saccharomyces; Streptococcus; 好氧生物反应器; 微生物组成; 微生物降解; 混合培养; 甜菜糖蜜; 通气条件; 酒糟
• ISSN: 1001-0742; 1878-7320
• DOI: 10.1016/S1001-0742(10)60579-7

The effect of aeration conditions and pH control on the progress and efficiency of beet molasses vinasse biodegradation was investigated during four batch processes at 38°C with the mixed microbial culture composed of Bifidobacterium,Lactobacillus,Lactococcus,Streptococcus,Bacillus,Rhodopseudomonas,and Saccharomyces.The four processes were carried out in a shake flask with no pH control,an aerobic bioreactor without mixing with no pH control,and a stirred-tank reactor （STR） with aeration with and without pH control,respectively.All experiments were started with an initial pH 8.0.The highest efficiency of biodegradation was achieved through the processes conducted in the STR,where betaine （an organic pollutant occurring in beet molasses in very large quantities） was completely degraded by the microorganisms.The process with no pH control carried out in the STR produced the highest reduction in the following pollution measures：organic matter expressed as chemical oxygen demand determined by the dichromatic method ＋ theoretical COD of betaine （COD sum,85.5%）,total organic carbon （TOC,78.8%） and five-day biological oxygen demand （BOD 5,98.6%）.The process conditions applied in the shake flask experiments,as well as those used in the aerobic bioreactor without mixing,failed to provide complete betaine assimilation.As a consequence,reduction in COD sum,TOC and BOD 5 was approximately half that obtained with STR.