In-vivo andex-vivo inhibition of intestinal glucose uptake：A scope for antihyperglycemia

• Published in: 急性病杂志（英文版） 2014 (1), p.36-40
• Main Authors: S Viviyan Therasa, T Thirumalai, N Tamilselvan, E David
• Format: Article
• Language: English
• ISSN: 2221-6189; 2221-6189
• DOI: 10.1016/S2221-6189(14)60008-1

Objective:To study hypoglycemic effect ofPhyllanthus amarus (P. amarus) leaf extract and its glucose uptake inhibition effect in rat small intestineex-vivo andin vivo models.Methods:Hypoglycemic studies were carried out in glucose loaded and streptozotocin(STZ) induced diabeticalbino rats.Blood glucose levels were estimated atI,III andIV hour time intervals after administration of aqueous leaf extract ofP. amarus.The study on the effect of plant extract on intestinal glucose absorption in rat was carried out using everted gut sacs.Results:The blood glucose levels were significantly depleted in the animals administered with aqueous leaf extract ofP. amarus(250 mg/kg body weight).Incubation of the rat everted intestinal sacs with the aqueous leaf extract ofP. amarus resulted in the inhibition of glucose transport across the intestinal membrane.Conclusions:The kinetic studies on the glucose transport inhibition across the intestinal membrane by the plant extract was a non competitive type of inhibition of the intestinal glucose transporter protein(GLUT2 andSGLT1) revealing the probable mechanism of hypoglycaemic effect of the aqueous leaf extract ofP. amarus.