Construction of chimeric inducible promoters by elicitors of rice fungal blast pathogen and their expression in transgenic rice

The promoter fragments of wheatGstA1 and potatoGst1 have been amplified by PCR, cloned and fused respectively to the minimal promoter sequence of rice actin gene (Act1)) and its 5′ untranslated leader sequence together withGUS. The constructs with 2 chimeric promoters (WGA and PGA) have been transfe...

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Bibliographic Details
Published in:Chinese science bulletin 2000-02, Vol.45 (3), p.242-246
Main Authors: Lü, Huafei, Ming, Xiaotian, Qu, Lijia, Liu, Meihua, Li, Jing, Gu, Hongya, Chen, Zhangliang
Format: Article
Language:English
Subjects:
Actin
Low level
Promoters
Rice
Rice blast
Transgenic plants
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Summary:The promoter fragments of wheatGstA1 and potatoGst1 have been amplified by PCR, cloned and fused respectively to the minimal promoter sequence of rice actin gene (Act1)) and its 5′ untranslated leader sequence together withGUS. The constructs with 2 chimeric promoters (WGA and PGA) have been transferred into rice in order to analyze their inducibility patterns in transgenic rice plants. The results show that: WGA and PGA are both inducible by elicitors ofPyricularia oryzae in transgenic rice cells; the intron I of riceAct1 gene is important for the heterogenic expression of monocot and dicot promoter elements in rice; and theAct1 minimal promoter and its 5′ untranslated leader sequence produced low level background expression in rice.
ISSN:1001-6538
2095-9273
1861-9541
2095-9281
DOI:10.1007/BF02884682