Depletion of phosphatidylglycerol head-group induces changes in oxygen evolution and protein secondary structures of photosystem

Q5; The techniques of oxygen electrode polarography and Fourier transform infrared (FT-IR) spectroscopy were employed to explore the roles of polar head-group of phosphatidylglycerol (PG) molecules in the functional and structural aspects of photosystemⅡ(PSⅡ) through enzymatic approach. It was shown...

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Published in:科学通报(英文版) 2002, Vol.47 (10), p.824-829
Main Authors: YANG Zhenle, WANG Zeneng, JIANG Guizhen, XU Yinong, LI Liangbi, KUANG Tingyun
Format: Article
Language:English
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Summary:Q5; The techniques of oxygen electrode polarography and Fourier transform infrared (FT-IR) spectroscopy were employed to explore the roles of polar head-group of phosphatidylglycerol (PG) molecules in the functional and structural aspects of photosystemⅡ(PSⅡ) through enzymatic approach. It was shown that the depletion of PG by treatment of phospholipase C (PLC) on PSⅡ particles caused the inhibition of oxygen evolving activity in PSⅡ. This effect also gave rise to changes in the protein secondary structures of PSⅡ, that is, an increase in α-helical conformation which is compensated by the loss of β-strand structures. It revealed that the head-group of PG molecules plays an important structural role in the maintenance of normal structure of PSⅡ proteins, which is required to maintain the appropriate physiological activity of the PSⅡ complex such as the oxygen evolving activity. It is suggested that there most probably exist hydrogen-bonding interactions between PG molecules and PSⅡ proteins.
ISSN:1001-6538
DOI:10.1360/02tb9186