Immobilization of biomacromolecules on poly-L-lactide surface via a layer-by-layer method for the improving of its cytocompatibility to bone marrow stromal cells

• Published in: Chinese science bulletin 2005-12, Vol.50 (24), p.2809-2816
• Main Authors: Lü, Delong, Meng, Sheng, Zhong, Wei, Du, Qiangguo, Gong, Li, Liu, Jinfen, Dusan, Bakos
• Format: Article
• Language: English
• Subjects: Amino groups; Biocompatibility; Bone marrow; Cell adhesion; Cell culture; Chitosan; Contact angle; Cytoplasm; Freeze drying; Hyaluronic acid; Immobilization; Leaching; Muscles; Polylactic acid; Pulmonary arteries; Pulmonary artery; Raman spectroscopy; Scaffolds; Smooth muscle; Spectroscopy; Spectrum analysis; Stromal cells; 壳聚糖; 生物材料; 细胞相容性; 聚-L-丙交酯; 表面改性; 透明质酸; 骨髓间质细胞
• ISSN: 1001-6538; 2095-9273; 1861-9541; 2095-9281
• DOI: 10.1360/982005-707

Hyaluronic acid （HA） and chitosan （CS） were immobilized on the surface of poly-L-lactide （PLLA） by the following procedure： Firstly, PLLA was aminolyzed with 1, 6-hexanediamine, and part of the PLLA surface ester groups were converted to free amino groups. Then negatively charged hyaluronic acid and positively charged chitosan were deposited onto the surface of aminolyzed PLLA film in a layer-by-layer assembly manner. The effect of the layer-by- layer deposition was evaluated by ATRoFTIR spectroscopy, Raman spectroscopy and static contact angle measurements. The cytocompatibility of PLLA sample to bone marrow stromal cells （BMSCs） was improved after modification with chitosan and HA. The cell attachment, activity, and proliferation on CS/HA modified PLLA films were enhanced comparing with the control. The cells cultured on the modified PLLA samples excreted abundant cytoplasm and can differentiate to vascular smooth muscle （SM）-like （SM-like） cells. A macroporous three-dimensional PLLA scaffold was prepared by integrating both the technique of freeze-drying and particle leaching. Layer-by-layer modification by HA/CS and cell culture was also applied on this scaffold. The scaffold cultured with BMSCs for 2 weeks has been tested successfully in vivo as a patch for repairing the artificial incision on canine pulmonary artery.