Rescued virus from infectious cDNA clone of rabbit hemorrhagic disease virus is adapted to RK13 cells line

Based on the infectious full-length cDNA clone of rabbit hemorrhagic disease virus （RHDV）, the in vitro transcripts are introduced into RK13 cells. 12 h later, CPE could be observed clearly, and virual antigen could also be detected by IFA. The titre of the recovered virus is 1046/mL. Immune electro...

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Bibliographic Details
Published in:Chinese science bulletin 2006-07, Vol.51 (14), p.1698-1702
Main Authors: Liu, Guangqing, Ni, Zheng, Yun, Tao, Zhang, Yuying, Du, Qingyun, Sheng, Zutian, Liang, Huali, Hua, Jionggang, Li, Shuangmao, Chen, Jianping
Format: Article
Language:English
Subjects:
Antigenicity
Antigens
cDNA
Cloning
Genetics
Hemorrhagic disease
Pathogenesis
Rabbit hemorrhagic disease
Rabbit hemorrhagic disease virus
RHDV
RK13细胞
Vaccines
Virulence
Viruses
兔出血性疾病
反求遗传学
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Summary:Based on the infectious full-length cDNA clone of rabbit hemorrhagic disease virus （RHDV）, the in vitro transcripts are introduced into RK13 cells. 12 h later, CPE could be observed clearly, and virual antigen could also be detected by IFA. The titre of the recovered virus is 1046/mL. Immune electron microscopic observation of the virus particles revealed that the particles were rotund with a diameter of about 30 nm. Besides, virus titre quantification obtained by qRT-PCR showed a correlation between time from infection and virus titre. All these results showed that we have recovered RHDV from RK13 cells by reverse genetics technology successfully, and this would be very useful in studies of the antigenicity, virulence, pathogenesis, maturation and new type vaccines of RHDV.
ISSN:1001-6538
2095-9273
1861-9541
2095-9281
DOI:10.1007/s11434-006-2044-x