Fluorescence Discrimination and Determination Technique for Phytoplankton Composition by Coif2 Wavelet Packet

• Published in: Journal of Ocean University of China 2013-03, Vol.12 (1), p.53-62
• Main Authors: Duan, Yali, Su, Rongguo, Shi, Xiaoyong, Wang, Xiulin, Zhu, Chenjian, Sun, Yan
• Format: Article
• Language: English
• Subjects: Algae; CDR; Community composition; Doctor Forum; Dominant species; Earth and Environmental Science; Earth Sciences; Fluorescence; Marine; Marine ecology; Meteorology; Oceanography; Phytoplankton; Plankton; Relative abundance; Water analysis; Water sampling; Wavelet transforms; 围隔实验; 小波包; 植物组成; 测定技术; 浮游植物; 聚类分析; 荧光技术
• ISSN: 1672-5182; 1993-5021; 1672-5174
• DOI: 10.1007/s11802-011-1902-9

An in vivo fluorescence discrimination technique for phytoplankton population was developed by using Wavelet packet transform, cluster analysis and non-negative least squares. The technique was used to analyze water samples from different sea regions. For simulative mixtures, when dominant species account for 60%, 70%, 80%, 90% at the division level, the correct discrimination ratios （CDRs） are 83.0%, 99.1%, 99.7% and 99.9% with the relative contents of 58.5%, 68.4%, 77.7% and 86.3%, respectively; when the algae dominance are 60%, 70%, 80%, 90%, 100% at the genus level, the CDRs are 86.1%, 94.9%, 95.2%, 96.8% and 96.7%, respectively. For laboratory mixtures, the CDRs are 88.1% and 78.4% at the division and genus level, respectively. For field samples, the CDRs were 91.7% and 80% at the division and genus level, respectively （mesocosm experiments）, and the CDRs were 100% and 66.7% at the division and genus level, respectively （Jiaozhou Bay）. The fluorometric technique was used to estimate the phytoplankton community composition and relative abundance of different classes for the April 2010 cruise in the Yellow Sea with the results agreeing with those in published papers by other authors.