Characterization and Functional Study on Octopus ocellatus Interleukin-17

Interleukin-17 (IL-17), a prototype member of IL-17 family, plays an important role in defending against extracellular pathogens as a pro-inflammatory cytokine. The function and distribution of IL-17 have been extensively studied in many vertebrates. However, few study has focused on the role of IL-...

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Bibliographic Details
Published in:Journal of Ocean University of China 2019-12, Vol.18 (6), p.1443-1450
Main Authors: Li, Zan, Fan, Tingting, Liu, Xintian, Liu, Xiumei, Wang, Weijun, Wang, Qianqian, You, Lunhe, Wang, Lei, Wei, Xiumei, Yang, Jianmin
Format: Article
Language:English
Subjects:
Cytokines
Defence mechanisms
DNA
Earth and Environmental Science
Earth Sciences
Extracellular
Gene expression
Gonads
Heart
Hemocytes
Hepatopancreas
Homology
Immune response
Immune system
Immunity
Inflammation
Interleukin 17
Interleukins
Invertebrates
Mantle
Marine molluscs
Meteorology
Mollusca
Mollusks
mRNA
Muscles
Nucleotide sequence
Oceanography
Pathogens
PCR
Phylogeny
Prototypes
Stomach
Tissue
Vertebrates
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Summary:Interleukin-17 (IL-17), a prototype member of IL-17 family, plays an important role in defending against extracellular pathogens as a pro-inflammatory cytokine. The function and distribution of IL-17 have been extensively studied in many vertebrates. However, few study has focused on the role of IL-17 in invertebrates, especially in mollusks. In this study, an IL-17 homolog was identified in Octopus ocellatus , which was designated as OoIL-17. The phylogenetic analysis showed that OoIL-17 is clustered well with other invertebrate IL-17, indicating it is highly similar with the IL-17 of other invertebrates. The expression of OoIL-17 gene was analyzed with qRT-PCR in a variety of healthy tissues and the hemocytes infected with Vibro anguillarum or Micrococcus luteus . The mRNA of OoIL-17 gene is constitutively expressed at different levels in all examined tissues of healthy O. ocellatus , including mantle, stomach, hemocytes, muscle, gonad, hepatopancreas, systemic heart and gill. The lowest expression was observed in mantle while the highest was observed in hepatopancreas. The expression level of OoIL-17 gene is significantly up-regulated in O. ocellatus hemocytes upon infection with V. anguillarum and M. luteus , indicating its active involvement in the host immune response against bacterial pathogens. The results laid the foundation for further understanding the innate immune mechanisms of IL-17 in O. ocellatus and mollusks.
ISSN:1672-5182
1993-5021
1672-5174
DOI:10.1007/s11802-019-4116-y