Extraction of Natural Nanostructured Hydroxyapatite from Pacific Cod (Gadus macrocephalus) Bone with a Thermostable Collagenolytic Protease and Its ex vivo Intestinal Bioavailability

Natural nano-hydroxyapatite (HA) was extracted from Pacific cod ( Gadus macrocephalus ) bone with a thermostable collagenolytic protease in the present study. Conditions for the enzymatic reaction were optimized to be 60 °C and pH 7.0, and a desirable extraction efficiency was achieved by using the...

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Bibliographic Details
Published in:Journal of Ocean University of China 2023-12, Vol.22 (6), p.1613-1620
Main Authors: Guo, Wei, Li, Shiyang, Jing, Zhehua, Wu, Haohao
Format: Article
Language:English
Subjects:
Amiloride
Anions
Bioavailability
Biomedical materials
Calcium
Calcium chloride
Clathrin
Collagen
Earth and Environmental Science
Earth Sciences
Electron microscopy
Endocytosis
Energy dispersive X ray analysis
Gadus macrocephalus
Hydroxyapatite
Intestinal absorption
Intestine
Light scattering
Low temperature
Meteorology
Natural resources
Oceanography
Phosphorus
Photon correlation spectroscopy
Protease
Sodium azide
Sodium azides
Transmission electron microscopy
X ray analysis
X-ray spectroscopy
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Summary:Natural nano-hydroxyapatite (HA) was extracted from Pacific cod ( Gadus macrocephalus ) bone with a thermostable collagenolytic protease in the present study. Conditions for the enzymatic reaction were optimized to be 60 °C and pH 7.0, and a desirable extraction efficiency was achieved by using the crude collagenolytic protease. Dynamic light scattering, transmission electron microscopy and energy-dispersive X-ray analysis revealed that nano-HA are anionic spherical (about 110 nm) particles mainly comprised of calcium and phosphorus at an approximate ratio of 5:3. As evaluated with the mouse ex vivo intestinal segments, the extracted nano-HA displayed comparable level of intestinal bioavailability to the positive control CaCl 2 . By treating with inhibitors (NaN 3 , amiloride) and low temperature (4°C), clathrin-mediated endocytosis was assumed to involve the intestinal absorption of nano-HA. Overall, the application of thermostable collagenolytic protease is proved to be a promising alternative method for nano-HA extraction from natural resource with improved ecological and biological value.
ISSN:1672-5182
1993-5021
1672-5174
DOI:10.1007/s11802-023-5589-2