Characterization and optimization of EPS- producing and diesel oil-degrading Ochrobactrum anthropi MP3 isolated from refinery wastewater

Petroleum refinery wastewater （PRW） containing hydrocarbon is highly toxic to the environment and the surrounding ecosystem. Proper treatment of the PRW effluent is necessary to remove the pollutants before discharge. Bioremediation is considered to be a promising approach as it is eco- friendly and...

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Bibliographic Details
Published in:Petroleum science 2014-09, Vol.11 (3), p.439-445
Main Authors: Ramasamy, Sugumar, Mathiyalagan, Prabhakaran, Chandran, Preethy
Format: Article
Language:English
Subjects:
Crude oil
Degradation
Diesel
Diesel fuels
Earth and Environmental Science
Earth Sciences
Economics and Management
Energy Policy
EPS
Hydrocarbons
Industrial and Production Engineering
Industrial Chemistry/Chemical Engineering
Mineral Resources
MP3
Ochrobactrum anthropi
Refineries
Waste water
优化生产
分离株
杆菌
柴油机
炼油废水
表征
降解
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Summary:Petroleum refinery wastewater （PRW） containing hydrocarbon is highly toxic to the environment and the surrounding ecosystem. Proper treatment of the PRW effluent is necessary to remove the pollutants before discharge. Bioremediation is considered to be a promising approach as it is eco- friendly and efficient. The exopolysaccharide （EPS） produced by the O. anthropi acts as a bioemulsifier and showed the highest emulsification activity of 60% on diesel. An EPS yield of about 0.42 g/L was obtained under optimized conditions. The carbohydrate and protein content of the EPS was found to be 71.1% and 19.7% respectively, showing the glycoprotein nature. The structural properties of EPS were analyzed by FT-IR and 1H NMR. The batch degradation of oil in PRW by O. anthropi was studied gravimetrically, and showed about 53% degradation in 7 days, indicating the strong ability of the isolated strain to degrade the hydrocarbons in PRW.
ISSN:1672-5107
1995-8226
DOI:10.1007/s12182-014-0359-9