Effect and Comparison of Sodium Butyrate and Trichostatin A on the Proliferation／Differentiation of K562

In order to explore the molecular mechanisms of sodium butyrate and trichostatin A on K562 cell proliferation/differentiation, K562 cells were grown in the absence or presence of sodium butyrate or trichostatin A. The percentage of viable cells was determined by trypan blue exclusion.Differentiation...

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Bibliographic Details
Published in:Current medical science 2003, Vol.23 (3), p.249-253
Main Author: 李春蕊 刘文励 孟凡凯 黄伟 周剑锋 孙汉英 冯永东
Format: Article
Language:English
Subjects:
Butyrates - pharmacology
Cell Cycle - drug effects
Cell Differentiation - drug effects
Cell Division - drug effects
Cyclin D
Cyclin-Dependent Kinase Inhibitor p21
Cyclins - biosynthesis
Cyclins - genetics
Gene Expression - drug effects
Histone Deacetylase Inhibitors
Humans
Hydroxamic Acids - pharmacology
K562 Cells
K562细胞
p21
TrichostatinA
丁酸钠
白血病
细胞周期蛋白D
细胞增殖
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Summary:In order to explore the molecular mechanisms of sodium butyrate and trichostatin A on K562 cell proliferation/differentiation, K562 cells were grown in the absence or presence of sodium butyrate or trichostatin A. The percentage of viable cells was determined by trypan blue exclusion.Differentiation was determined by nitro-blue tetrazolium (NBT) reduction and cell surface adhesion molecules analyzed by FACS. Cell cycle distribution was studied after DNA staining by propidium iodide. Cell cycle regulatory proteins were detected by Western blot and reverse transcription-poly-merase chain reaction. The results showed that sodiun butyrate blocked ceils mainly at the G0/G1 phase of the cell cycle, whereas trichostatin A arrested the cells at G2 phase. Sodium butyrate coulddown-regulate the mRNA expression of cyclin D1, but not affect its protein expression; down-regu-late the protein expression of cyclin D3, but not affect its mRNA expression. Trichostatin A showed similar effects on cyclin D1 and D3 as sodium butyrate. Both sodium butyrate and trichostatin A could stimulate p21 expression of K562 cells at mRNA and protein levels. It may be concluded that sodium butyrate and trichostatin A could promote the proliferation/differentiation of the K562 cells, which might be contributed to the induced expression of cyclin D3 and D21 proteins.
ISSN:1672-0733
2096-5230
1993-1352
2523-899X
DOI:10.1007/bf02829505