Expression of CD28 and CTLA4 on T Cells in Bone Morrow of Immune-mediated Aplastic Anemia Mice

• Published in: Journal of Huazhong University of Science and Technology. Medical sciences 2005, Vol.25 (5), p.508-509
• Main Author: 刘振芳 孙汉英 刘文励 罗小华 何莉 徐慧珍
• Format: Article
• Language: English
• Subjects: Anemia, Aplastic - immunology; Anemia, Aplastic - metabolism; Animals; Antigens, CD - genetics; Antigens, CD - metabolism; Bone Marrow Cells - immunology; Bone Marrow Cells - metabolism; CD28; CD28 Antigens - genetics; CD28 Antigens - metabolism; CTLA-4 Antigen; CTLA4; Female; Male; Mice; Mice, Inbred BALB C; T-Lymphocytes - immunology; T-Lymphocytes - metabolism; T细胞; 免疫调节; 动物实验; 基因表达
• ISSN: 1672-0733; 1993-1352
• DOI: 10.1007/bf02896001

Summary： To investigate the expression and significance of CD28 and CTLA4 on T cells in bone marrow of aplastic anemia （AA） mice, in vitro bone marrow mononuclear cells （BMMNCs） were activated through being incubated with PHA （15 μg/mL）. The expression of CD28 and CTLA4 on T cells incubated with or without PHA was detected by two-color flow cytometry. The expression of CD28 and CTLA4 was significantly increased after PHA stimulation. In the AA mice. the expression of CD28 with or without PHA stimulation was both higher than that in the normal mice （both P〈0.01）, but the expression of CTLA4 with or without PHA stimulation showed no significant difference in comparison to that in the normal mice （both P〉0.05）. In the AA mice, there were more activation and activated potential of T cells than the normal, and the abnormal expression of CD28 and CTLA4 may participate in immunological disorder mediated by T cells.