Cyclin E Expression and Chemotherapeutic Sensitivity in Breast Cancer Cells

The effects of the cyclin E expression levels on chemotherapeutic sensitivity of breast cancer cell line were explored. After the cyclin E expression was knockdown in MDA-MB-435 by RNA interference, FACS analysis and SA-β-gal staining were used to evaluate the response sensitivity of breast cancer c...

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Bibliographic Details
Published in:Journal of Huazhong University of Science and Technology. Medical sciences 2006, Vol.26 (5), p.565-566
Main Author: 陈剑英 王国斌
Format: Article
Language:English
Subjects:
Antibiotics, Antineoplastic - pharmacology
Breast Neoplasms - metabolism
Breast Neoplasms - pathology
Cell Line, Tumor
Cellular Senescence
Cyclin E - genetics
Cyclin E - metabolism
DNA Damage - drug effects
Doxorubicin - pharmacology
Gene Knockdown Techniques
Humans
Oncogene Proteins - genetics
Oncogene Proteins - metabolism
RNA Interference
乳腺癌
化学治疗
基因表达
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Summary:The effects of the cyclin E expression levels on chemotherapeutic sensitivity of breast cancer cell line were explored. After the cyclin E expression was knockdown in MDA-MB-435 by RNA interference, FACS analysis and SA-β-gal staining were used to evaluate the response sensitivity of breast cancer cells to DNA damage drugs （adriamycin, etc.）. Adriamycin could induce G1 arrest in cyclin E knockdown MDA-MB-435 breast cell line and increase the percentage of cell senescence in cyclin E knockdown MDA-MB-435 cells. It was suggested that cyclin E knockdown could increase the chemotherapeutic sensitivity of breast cancer cells to DNA damage drugs.
ISSN:1672-0733
1993-1352
DOI:10.1007/s11596-006-0521-8