Altered expression of connexin-43 and impaired capacity of gap junctional intercellular communication in prostate cancer cells

Connexin-43 (Cx43) expression in prostate cancer (PCa) cells and the potency of gap junctional intercellular communication (GJIC) in the cells were investigated, with an attempt to elucidate the reason why the so-called "bystander effect" mediated by thymidine kinase (TK) suicide gene ther...

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Bibliographic Details
Published in:Journal of Huazhong University of Science and Technology. Medical sciences 2007-06, Vol.27 (3), p.291-294
Main Authors: Xing, Yifei, Xiao, Yajun, Zeng, FuQing, Zhao, Jun, Xiao, Chuanguo, Xiong, Ping, Feng, Wei
Format: Article
Language:English
Subjects:
Cell Communication
Cell Line, Tumor
Connexin 43 - biosynthesis
Connexin 43 - genetics
Gap Junctions - metabolism
Gene Expression Regulation, Neoplastic
Humans
Immunohistochemistry
Male
Prostatic Neoplasms - genetics
Prostatic Neoplasms - metabolism
Prostatic Neoplasms - pathology
Reverse Transcriptase Polymerase Chain Reaction
RNA, Messenger - biosynthesis
RNA, Messenger - genetics
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Summary:Connexin-43 (Cx43) expression in prostate cancer (PCa) cells and the potency of gap junctional intercellular communication (GJIC) in the cells were investigated, with an attempt to elucidate the reason why the so-called "bystander effect" mediated by thymidine kinase (TK) suicide gene therapy on PCa cells is not of significance and to explore the role of GJIC in PCa carcinogenesis. mRNA and protein expression of Cx43 in a PCa cell line PC-3m was detected by reverse-transcription polymerase chain reaction (RT-PCR) and strapt-avidin-biotin-enzyme complex (SABC) immunohistochemical staining, and inherent GJIC of PC-3m cells was assayed by scrape-loading and dye transfer (SLDT) assay. The expression of Cx43 in human normal and malignant prostate tissues was determined by SABC immunohistochemistry as well. It was found that Cx43 mRNA and protein expression in PC-3m cells was slightly reduced as compared with positive controls and the location of Cx43 protein was aberrant in cytoplasm rather than on membrane. Assessment of paraffin sections demonstrated that the expression of Cx43 protein in PCa cells was abnormally located and markedly diminished as compared with normal prostatic epithelial ones, displaying a negative correlation to the pathological grade (chi2=4.025, P
ISSN:1672-0733
1993-1352
DOI:10.1007/s11596-007-0319-3