Screening of Differently Expressed Genes in Human Prostate Cancer Cell Lines with Different Metastasis Potentials

• Published in: Journal of Huazhong University of Science and Technology. Medical sciences 2007-10, Vol.27 (5), p.582-585
• Main Author: 宋安萍 廖国宁 吴明富 卢运萍 马丁
• Format: Article
• Language: English
• Subjects: Cell Line, Tumor; Gene Expression Profiling; Gene Expression Regulation, Neoplastic; Gene Library; Humans; Male; Neoplasm Metastasis - genetics; Nucleic Acid Hybridization - methods; Prostatic Neoplasms - genetics; Prostatic Neoplasms - pathology; 前列腺癌; 基因表达; 抑制减数杂交; 肿瘤转移
• ISSN: 1672-0733; 1993-1352
• DOI: 10.1007/s11596-007-0527-x

In order to screen the genes differentially expressed in two human prostate cancer cells with different metastasis potentials, suppression subtractive hybridization （SSH） was done twice on human prostate cancer cell line with high potential of metastasis PC3M-IE8 and its synogenetic cell line PC3M-2B4 with low metastasis potential. In the first subtraction PC3M-2B4 was used as tester and PC3M-1E8 as driver and the forward subtractive library was constructed. In the second one the tester and driver were interchanged and the reverse subtractive library was constructed. The screened clones of both libraries were sequenced and Gene Bank homology search was performed. Some clones were confirmed by quantitative real-time PCR. The results showed that two subtractive libraries containing 238 positive clones were constructed. Analysis of 16 sequenced clones randomly picked from two libraries showed that 4 differentially expressed gene fragments were identified as new EST with unknown functions. It was concluded that two subtractive libraries of human prostate cancer cell lines with different metastasis potentials were constructed successfully.