Astrocytes Facilitate the Growth and Differentiation of Co-cultured Mesenchymal Stem Cells

The effect of astrocytes on the growth and differentiation of co-cultured mesenchymal stem cells （MSCs） was studied. MSCs of Wistar rats were isolated, cultured and labeled with Hoechst33342. The labeled MSCs were co-cultured with astrocytes in DMEM/F12/10%FBS. The growth status and morphologic chan...

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Bibliographic Details
Published in:Journal of Huazhong University of Science and Technology. Medical sciences 2008-06, Vol.28 (3), p.333-336
Main Author: 褚倩 喻志源 张苏明 于世英
Format: Article
Language:English
Subjects:
Animals
Astrocytes - cytology
Cell Differentiation
Cell Proliferation
Cell Transplantation
Coculture Techniques - methods
Glial Fibrillary Acidic Protein - metabolism
Immunohistochemistry - methods
Medicine
Medicine & Public Health
Mesenchymal Stromal Cells - cytology
Microscopy, Fluorescence - methods
Neuroglia - cytology
Neurons - metabolism
Rats
Rats, Wistar
Stem Cells - cytology
星形胶质细胞
神经细胞
间叶干细胞
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Summary:The effect of astrocytes on the growth and differentiation of co-cultured mesenchymal stem cells （MSCs） was studied. MSCs of Wistar rats were isolated, cultured and labeled with Hoechst33342. The labeled MSCs were co-cultured with astrocytes in DMEM/F12/10%FBS. The growth status and morphologic change of MSCs were observed. The expression of specific protein NeuN, GFAP and CNP was detected by using immunofluorescence staining. The results showed that the two kinds of co-cultured cells grew well. Some labeled MSCs stretched out long processes with spin, triangle and star shapes. Immunofluorescence stain showed that these cells expressed NeuN, GFAP or CNP. It was suggested that astrocytes could promote the prolifevation of co-cultured MSCs and induce them to differentiate into neural like cells.
ISSN:1672-0733
1993-1352
DOI:10.1007/s11596-008-0323-2