Immune Killing Activity of Lymphocytes on Hela Cells Expressing Interleukin-12 In Vitro

The killing effects of lymphocytes on Hela cells expressing interleukin-12 （IL-12） in vitro were explored. By using gene transfection technique, full length IL-12 gene was transfected into Hela cells. The expression of IL-12 in Hela cells was detected quantitatively by ELISA; Changes in killing effe...

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Bibliographic Details
Published in:Journal of Huazhong University of Science and Technology. Medical sciences 2008-06, Vol.28 (3), p.343-345
Main Author: 王慧燕 陈素华
Format: Article
Language:English
Subjects:
Apoptosis
Cell Survival
Coculture Techniques
Female
Gene Expression Regulation, Neoplastic
HeLa Cells
Humans
Immune System
Interleukin-12 - biosynthesis
Interleukin-12 - metabolism
Interleukin-12 - physiology
Killer Cells, Natural - cytology
Lymphocytes - metabolism
Lymphocytes - pathology
Medicine
Medicine & Public Health
T-Lymphocytes, Cytotoxic - cytology
Tetrazolium Salts - pharmacology
Thiazoles - pharmacology
Time Factors
Uterine Cervical Neoplasms - metabolism
免疫杀伤力活性
海拉细胞
淋巴细胞
白介素-12
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Summary:The killing effects of lymphocytes on Hela cells expressing interleukin-12 （IL-12） in vitro were explored. By using gene transfection technique, full length IL-12 gene was transfected into Hela cells. The expression of IL-12 in Hela cells was detected quantitatively by ELISA; Changes in killing effects of lymphocytes on Hela cells expressing IL-12 were observed by MTT. It was found that Hela cells could express IL- 12 between 24 h and 72 h after transfection. Killing activity of lymphocytes on Hela cells expressing IL-12 was significantly enhanced. It was concluded by cell transfection technique, Hela cells could express 1L-12 and were more easily killed by lymphocytes.
ISSN:1672-0733
1993-1352
DOI:10.1007/s11596-008-0326-z