Rapamycin Modulates the Maturation of Rat Bone Marrow-derived Dendritic Cells

The purpose of the study was to observe the effect of rapamycin （RAPA） on the differentiation and maturation of rat bone marrow-derived dendritic cells （BMDCs） in vitro. BMDCs from Wistar rats were cultured with granulocyte-macrophage colony-stimulating factor plus interleukin-4 in the presence or a...

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Published in:Journal of Huazhong University of Science and Technology. Medical sciences 2008-08, Vol.28 (4), p.391-395
Main Author: 丁英俊 程翔 唐婷婷 姚瑞 陈勇 谢江娇 余娴 廖玉华
Format: Article
Language:English
Subjects:
Animals
BMDCs
Bone Marrow Cells - cytology
Cell Differentiation - drug effects
Cell Differentiation - physiology
Cells, Cultured
Dendritic Cells - cytology
Granulocyte-Macrophage Colony-Stimulating Factor - pharmacology
Interleukin-4 - pharmacology
Lipopolysaccharides - pharmacology
Male
Medicine
Medicine & Public Health
Rats
Rats, Wistar
Sirolimus - pharmacology
树突细胞
脂多糖
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description The purpose of the study was to observe the effect of rapamycin （RAPA） on the differentiation and maturation of rat bone marrow-derived dendritic cells （BMDCs） in vitro. BMDCs from Wistar rats were cultured with granulocyte-macrophage colony-stimulating factor plus interleukin-4 in the presence or absence of RAPA （20 ng/mL）, and stimulated with lipopolysaccharide （LPS） for 24 h before cells and supernatants were collected. Surface phenotype of BMDCs was flow-cytometrically detected to determine the expression of maturation markers, MHC class Ⅱ and CD86. Supernatants were analyzed for the production of IL-12 and IFN-γ cytokines by using ELISA. BMDCs were co-cultured with T cells from Lewis rats and mixed lymphocyte reaction was assessed by MTT method. The morphology of BMDCs stimulated with LPS remained immature after RAPA pretreatment. RAPA significantly decreased the CD86 expression, impaired the IL-12 and IFN-γ production of BMDCs stimulated with LPS, and inhibited the proliferation of allogeneic T cells. In conclusion, RAPA can inhibit the maturation of BMDCs stimulated with LPS in terms of the morphology, surface phenotype, cytokine production, and ability of BMDCs to stimulate the proliferation of allogeneic T cells in vitro.
doi_str_mv 10.1007/s11596-008-0405-1
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identifier ISSN: 1672-0733
ispartof Journal of Huazhong University of Science and Technology. Medical sciences, 2008-08, Vol.28 (4), p.391-395
issn 1672-0733
1993-1352
language eng
recordid cdi_wanfang_journals_tjykdxxb_e200804005
source Springer Nature
subjects Animals
BMDCs
Bone Marrow Cells - cytology
Cell Differentiation - drug effects
Cell Differentiation - physiology
Cells, Cultured
Dendritic Cells - cytology
Granulocyte-Macrophage Colony-Stimulating Factor - pharmacology
Interleukin-4 - pharmacology
Lipopolysaccharides - pharmacology
Male
Medicine
Medicine & Public Health
Rats
Rats, Wistar
Sirolimus - pharmacology
树突细胞
脂多糖
title Rapamycin Modulates the Maturation of Rat Bone Marrow-derived Dendritic Cells
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