Effect of the artificial somato-autonomic neuroanastomosis on defecation after spinal cord injury and its underlying mechanisms

Summary A new artificial somatic-autonomic neuroanastomosis has been established in male rats with spinal cord injury (SCI). Anorectal manometry and neural retrograde tracing were conducted in this animal model to analyze the mechanisms and the effects on recovery of anorectal function. The left L4...

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Bibliographic Details
Published in:Journal of Huazhong University of Science and Technology. Medical sciences 2010-08, Vol.30 (4), p.490-493
Main Authors: Sun, Fengyin, Chen, Min, Li, Wencheng, Xiao, Chuanguo
Format: Article
Language:English
Subjects:
Anastomosis, Surgical - methods
Angulus
Animals
Autonomic Nervous System - surgery
Constipation - etiology
Defecation - physiology
Male
Medicine
Medicine & Public Health
Nerve Regeneration
Neurogenic Bowel - etiology
Neurogenic Bowel - surgery
Neurosurgical Procedures - methods
Rats
Rats, Sprague-Dawley
Spinal Cord Injuries - complications
Spinal Nerve Roots - surgery
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Summary:Summary A new artificial somatic-autonomic neuroanastomosis has been established in male rats with spinal cord injury (SCI). Anorectal manometry and neural retrograde tracing were conducted in this animal model to analyze the mechanisms and the effects on recovery of anorectal function. The left L4 ventral root (L4VR) was intradurally micro-anastomosed to the L6 ventral root (L6VR) to establish the new regenerated neural pathway. Three months later the spinal cord was completely transected at the T9–10 level. Eight weeks later the model rats were randomly divided into two groups. The rats in the group 1 ( n =8) were applied for anorectal manometry, and those in the group 2 ( n =4) were used for neural retrograde tracing study with fluorogold (FG) and dextran tetramethylrhodamine (TMR). The results of anorectal manometry showed the new reflex pathway could induce rectum to contract and simultaneously electric activity of external anal sphincter (EAS) to become weak or disappearing (indicating synergetic relaxation of EAS). FG and TMR dual labeled neurons with round and elliptical shape were mainly observed in L4 angulus anterior of model rats. The regenerated neural pathways were effective to improve the rectum external sphincter synergetic status and restore the anorectal function.
ISSN:1672-0733
1993-1352
DOI:10.1007/s11596-010-0455-z