Neurogenesis of Adipose-derived Stem Cells in Hydrogel

Adipose tissue is a readily available source of adult stem cells with multipotent properties suitable for tissue engineering and regenerative medical applications. Peptide hydrogel is a novel biomaterial which provides three-dimensional microenvironments for a variety of cells for tissue grafting. I...

Full description

Saved in:
Bibliographic Details
Published in:Journal of Huazhong University of Science and Technology. Medical sciences 2011-04, Vol.31 (2), p.174-177
Main Author: 谢雪微 唐洲平 陈娟 杨洁 曾文高 刘娜 刘永明
Format: Article
Language:English
Subjects:
Adipose Tissue - cytology
Animals
Cell Differentiation - physiology
Hydrogels - chemistry
Male
Medicine
Medicine & Public Health
Neurons - cytology
Rats
Rats, Sprague-Dawley
Stem Cells - cytology
Tissue Engineering
Tissue Scaffolds
凝胶聚合物
医学应用
无性系种群
水凝胶
碱性成纤维细胞生长因子
神经再生
脂肪干细胞
表皮生长因子
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Adipose tissue is a readily available source of adult stem cells with multipotent properties suitable for tissue engineering and regenerative medical applications. Peptide hydrogel is a novel biomaterial which provides three-dimensional microenvironments for a variety of cells for tissue grafting. In this study, adipose-derived stem cells （ADSCs） were isolated from rats, seeded into the peptide hydrogel polymer scaffolds and cultured in Neurobasal （NB） media supplemented with B27, basic fibroblast growth factor （bFGF） and epidermal growth factor （EGF）. Ten days after the culture, some cells were expanded into clonal populations in which the expression of both Nestin and Brdu was detected but only Brdu expression was detected in the cells that were not expanded into clonal populations. Our results suggested that ADSCs in peptide hydrogel polymer scaffolds can be induced to differentiate into cells capable of expressing the neuron-associated markers, self-renewal and self-propagation.
ISSN:1672-0733
1993-1352
DOI:10.1007/s11596-011-0246-1