Over-expression of LRIG3 Suppresses Growth and Invasion of Bladder Cancer Cells

The purpose of this study was to investigate the impact of leucine-rich repeats and immu- noglobulin-like domains 3 （LRIG3） on the biological features of bladder cancer cell lines. The plasmids of over-expressed LRIG3 and the blank plasmid serving as control were transfected into the bladder cancer...

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Published in:Journal of Huazhong University of Science and Technology. Medical sciences 2013-02, Vol.33 (1), p.111-116
Main Author: 齐永 昌磊 李恒 余淦 肖巍 夏丁 管维 杨洋 郎槟 邓康俐 姚炜敏 叶章群 庄乾元
Format: Article
Language:English
Subjects:
Apoptosis
Cell Line, Tumor
Cell Movement
Cell Proliferation
Humans
Medicine
Medicine & Public Health
Membrane Proteins - genetics
Membrane Proteins - metabolism
Neoplasm Invasiveness
Up-Regulation
Urinary Bladder Neoplasms - metabolism
Urinary Bladder Neoplasms - pathology
Western印迹
侵袭
免疫球蛋白
富含亮氨酸重复序列
流式细胞术检测
癌细胞
膀胱癌
过表达
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Summary:The purpose of this study was to investigate the impact of leucine-rich repeats and immu- noglobulin-like domains 3 （LRIG3） on the biological features of bladder cancer cell lines. The plasmids of over-expressed LRIG3 and the blank plasmid serving as control were transfected into the bladder cancer cell lines, T24, EJ and BIU-87, and the expression levels of LRIG3 mRNA and protein were de- tected by using real-time PCR and Western blotting. The changes in the cell cycle and apoptosis were examined by using flow cytometry. The invasive ability was measured by Transwell assay, and CCK-8 assays were used to measure the proliferation of cells. As compared with the control group, the LRIG3 mRNA and protein expression levels in LRIG3 cDNA-transfected group were raised significantly （P〈0.05）. The average number of cells with up-regulated LRIG3 passing through the inserted filter was decreased significantly as compared with the control group （P〈0.05）. Up-regulation of LRIG3 also could inhibit proliferation and induce apoptosis of T24, EJ and BIU-87 cells. Except BIU-87, the T24 and EJ cells transfected with LIRG3 eDNA were arrested in G0/G1 phase compared to the control group （P〈0.05）. In conclusion, the over-expression of LRIG3 could influence the cell cycle and invasion, in- hibit proliferation and induce apoptosis in the three bladder cancer cell lines.
ISSN:1672-0733
1993-1352
DOI:10.1007/s11596-013-1081-3