Correlation between Increased Circulating Endothelial Progenitor Cells and Stage of non-Hodgkin Lymphoma

• Published in: Journal of Huazhong University of Science and Technology. Medical sciences 2013-04, Vol.33 (2), p.284-287
• Main Author: 于丹丹 刘红利 白云玲 吴边 陈卫红 任精华 张涛 杨坤禹 伍钢
• Format: Article
• Language: English
• Subjects: Blood Cell Count; Cells, Cultured; Endothelial Cells - pathology; Female; Humans; Lymphoma, Non-Hodgkin - blood; Lymphoma, Non-Hodgkin - pathology; Male; Medicine; Medicine & Public Health; Neoplastic Cells, Circulating - pathology; Statistics as Topic; Stem Cells - pathology; 内皮祖细胞; 外周血单个核细胞; 密度梯度离心; 循环; 流式细胞仪分析; 淋巴瘤; 血管内皮生长因子受体; 金
• ISSN: 1672-0733; 1993-1352
• DOI: 10.1007/s11596-013-1112-0

This study aims to examine the levels of circulating endothelial progenitor cells （cEPCs） in the peripheral blood of patients with non-Hodgkin lymphoma （NHL） and their correlation with the tumor stage. Forty-one patients with biopsy-proven NHL and 16 healthy individuals were recruited. Pe- ripheral blood mononuclear cells （PBMCs） were isolated by density gradient centrifugation, and cEPCs were characterized by triple staining using antibodies against CD133, CD34 and vascular endothelial growth factor receptor-2 （VEGFR-2, CD309） and quantified by flow cytometry. In NHL patients, the number of cEPCs was significantly greater than in control group （P=-0.000）. The cEPCs counts in patients with NHL of stage III-1V were significantly greater than in stage I -II （P=-0.010）. FACS analysis revealed that the number of cEPCs in NHL patients had no correlation with the gender （P=0.401） or the pathological category （P=0.852）. It was suggested that the over-expression of cEPCs in NHL patients may serve as a novel biomarker for disease progression in NHL.