Effects of IL-17 on Expression of GRO-α and IL-8 in Fibroblasts from Nasal Polyps

• Published in: Journal of Huazhong University of Science and Technology. Medical sciences 2014-08, Vol.34 (4), p.591-595
• Main Author: 牛永芝 龚国清 陈删 陈建军 孔维佳 王彦君
• Format: Article
• Language: English
• Subjects: Adult; Cells, Cultured; Chemokine CXCL1 - biosynthesis; Female; Fibroblasts - metabolism; Fibroblasts - pathology; Humans; IL-17; IL-8; Interleukin-17 - pharmacology; Interleukin-8 - biosynthesis; Male; Medicine; Medicine & Public Health; Middle Aged; Nasal Polyps - metabolism; Nasal Polyps - pathology; Neutrophil Infiltration - drug effects; RNA, Messenger - biosynthesis; RT-PCR; 成纤维细胞; 细胞浸润; 肉; 荧光定量聚合酶链反应; 鼻炎
• ISSN: 1672-0733; 1993-1352
• DOI: 10.1007/s11596-014-1321-1

Recent studies indicated that interleukin（IL）-17, growth-related oncogene（GRO）-α and IL-8 play an important role in the pathogenesis of nasal polyps. However, the effects of the increased amount of IL-17 and the production of GRO-α and IL-8 in human nasal polyp fibroblasts are not completely understood. This study aimed to determine the effects of the increased IL-17 on the changes of GRO-α and IL-8 expression in human nasal polyp fibroblasts and further investigate the mechanism of neutrophil infiltration in nasal polyps. Nasal polyp fibroblasts were isolated from six cases of human nasal polyps, and the cells were stimulated with five different concentrations of IL-17. Real-time fluorescence quantitative polymerase chain reaction（RT-PCR） was used to detect the mRNA expression of GRO-α and IL-8. The mRNA of GRO-α and IL-8 was expressed in unstimulated controls and remarkably increased by stimulation with IL-17. Moreover, the levels of GRO-α and IL-8 produced by fibroblasts were increased gradually with the increases in IL-17 concentrations. The present study showed that nasal fibroblasts can produce GRO-α and IL-8, and their production is remarkably enhanced by IL-17 stimulation, thereby clarifying the mechanism of the IL-17 mediated neutrophil infiltration in nasal polyps. These findings might provide a rationale for using IL-17 inhibitors as a treatment for nasal inflammatory diseases such as nasal polyps.