Effects of Bu-Shen-An-Tai Recipe and Its Two Components on Endometrial Morphology during Peri-implantation in Superovulated Mice

• Published in: Journal of Huazhong University of Science and Technology. Medical sciences 2014-10, Vol.34 (5), p.768-774
• Main Author: 崔丹丹 郑翠红 龚萍 文露 马雯雯 周顺长 张明敏
• Format: Article
• Language: English
• Subjects: Animals; Antigens, CD34 - metabolism; Drugs, Chinese Herbal - chemistry; Drugs, Chinese Herbal - pharmacology; Embryo Implantation - physiology; Endometrium - drug effects; Endometrium - physiology; Endometrium - ultrastructure; Female; Immunohistochemistry; Medicine; Medicine & Public Health; Mice; Microscopy, Electron, Scanning; Microvessels - metabolism; Microvessels - physiology; Ovulation - physiology; Pregnancy; Random Allocation; Time Factors; 子宫内膜; 小鼠; 微血管密度; 植入; 膜厚度; 膜形态; 补肾; 超数排卵
• ISSN: 1672-0733; 1993-1352
• DOI: 10.1007/s11596-014-1350-9

The aim of this study was to investigate the effect of Bu-Shen-An-Tai recipe （BSATR） and its two components （Bushen recipe, and Huoxue recipe） on endometrial morphology during peri-implantation in superovulated mice. Mice were randomly divided into five groups, including the normal （N）, model （M）, Bushen （BS）, Huoxue （HX） and Bu-Shen-An-Tai （BH） groups. The uteri were collected on day 4 of pregnancy, and the endometrium thickness, microvessel density （MVD） and number of pinopodes observed. Compared with the M group, the endometrial thickness in the BS, HX and BH groups was significantly increased and there was a significant difference in endometrial thickness between the BS and the BH groups. The mean MVD was significantly lower in the M group than in the N group, and there was a significant increase in MVD in the BS, HX and BH groups as compared with the M group. Compared with the M group, the pinopode scores in the en- dometrium were significantly increased in the HX and BH groups; and the BS group had significantly higher pinipode scores than the HX and BH groups. In conclusion, the results of the present study demonstrated that the recipes （Bushen, Huoxue and BSATR） could improve the endometrial envi- ronment by regulating the endometrial thickness, MVD and the number of pinopodes at the window of implantation. Moreover, the Huoxue recipe and the BSATR were more efficient than the Bushen recipe, with the BSATR tending to have the most beneficial effects.