Characterization of Anabaena cylindrica Solution System Using Synchronous-Scan Fluorescence Spectroscopy

• Published in: Wuhan University journal of natural sciences 2005-03, Vol.10 (2), p.441-447
• Main Authors: Liu, Xian-li, Deng, Nan-sheng, Tao, Shu
• Format: Article
• Language: English
• ISSN: 1007-1202
• DOI: 10.1007/bf02830684

The characterization of the algae Anabaena cylindrica solution with Fe (III) was investigated using fluorescence emission and synchronous-scan spectroscopy. The ranges of concentrations of algae and Fe (III) in aqueous solutions were 5. 0 X 10 super(7)-2. 5 X 10 super(8) cell/L and 10-60 mu mol/L, respectively. The effective characterization method used was synchronous-scan fluorescence spectroscopy (SFS). The wavelength difference ( Delta lambda ) of 90 nm was maintained between excitation wavelength ( lambda sub(ex)) and emission wavelength( lambda sub(em)). The peak was observed at about lambda sub(ex) 236 nm/ lambda sub(em) 326 nm for synchronous-scan fluorescence spectroscopy. The fluorescence quenching in system of algae-Fe(III)-HA was studied using synchronous-scan spectroscopy for the first time. Fe(III) was clearly the effective quencher. The relationship between I sub(0)/I (quenching efficiency) and c (concentration of Fe (III) added) was a linear correlation for the algae solution with Fe(III). Also, Aldrich humic acid (HA) was found to be an effective quencher.