Protective effects of erythropoietin against acute lung injury in a rat model of acute necrotizing pancreatitis

• Published in: World journal of gastroenterology : WJG 2007-12, Vol.13 (46), p.6172-6182
• Main Authors: Tascilar, Oge, Cakmak, Güldeniz Karadeniz, Tekin, Ishak Ozel, Emre, Ali Ugur, Ucan, Bulent Hamdi, Bahadir, Burak, Acikgoz, Serefden, Irkorucu, Oktay, Karakaya, Kemal, Balbaloglu, Hakan, Kertis, Gurkan, Ankarali, Handan, Comert, Mustafa
• Format: Article
• Language: English
• Subjects: Amylases - blood; Animals; Body Weight; Disease Models, Animal; Erythropoietin - pharmacology; Interleukin-2 - blood; Interleukin-6 - blood; Lipoproteins, LDL - metabolism; Lung - metabolism; Lung - pathology; Male; Malondialdehyde - metabolism; Neutrophils - pathology; Pancreatitis, Acute Necrotizing - chemically induced; Pancreatitis, Acute Necrotizing - complications; Pleural Effusion; Pulmonary Alveoli - pathology; Rats; Rats, Wistar; Respiratory Distress Syndrome, Adult - etiology; Respiratory Distress Syndrome, Adult - pathology; Respiratory Distress Syndrome, Adult - prevention & control; Taurodeoxycholic Acid; 急性期物质; 急性肺炎; 红血球; 细胞因子; 胰腺炎
• ISSN: 1007-9327; 2219-2840
• DOI: 10.3748/wjg.13.6172

AIM： TO investigate the effect of exogenous erythro- poietin （EPO） administration on acute lung injury （ALI） in an experimental model of sodium taurodeoxycholate- induced acute necrotizing pancreatitis （ANP）. METHODS： Forty-seven male Wistar albino rats were randomly divided into 7 groups： sham group （n = 5）, 3 ANP groups （n = 7 each） and 3 EPO groups （n = 7 each）. ANP was induced by retrograde infusion of 5% sodium taurodeoxycholate into the common bile duct. Rats in EPO groups received 1000 U/kg intramuscular EPO immediately after induction of ANP. Rats in ANP groups were given 1 mL normal saline instead. All animals were sacrificed at postoperative 24 h, 48 h and 72 h. Serum arnilase, IL-2, IL-6 and lung tissue malondialdehyde （MDA） were measured. Pleural effusion volume and lung/body weight （LW/BW） ratios were calculated. Tissue levels of TNF-a, IL-2 and IL-6 were screened immunohistochemically. Additionally, ox-LDL accumulation was assessed with immune-fluorescent staining. Histopathological alterations in the lungs were also scored.RESULTS： The mean pleural effusion volume, calculated LW/BW ratio, serum IL-6 and lung tissue MDA levels were significantly lower in EPO groups than in ANP groups. No statistically significant difference was observed in either serum or tissue values of IL-2 among the groups. The level of tumor necrosis factor-（~ （TNF-（~） and IL-6 and accumulation of ox-LDL were evident in the lung tissues of ANP groups when compared to EPO groups, particularly at 72 h. Histopathological evaluation confirmed the improvement in lung injury parameters a~er exogenous EPO administration, particularly at 48 h and 72 h. CONCLUSION： EPO administration leads to a significant decrease in ALI parameters by inhibiting polymorphonuclear leukocyte （PMNL） accumulation, decreasing the levels of proinflammatory cytokines in circulation, preserving microvascular endothelial cell integrity and reducing oxidative stress-associated lipid peroxidation and therefore, can be regarded as a cytoprotective agent in ANP-induced ALI.