A nuclear localized protein ZCCHC9 is expressed in cerebral cortex and suppresses the MAPK signal pathway

The CCHC-type zinc finger motif has numerous biological activities （such as DNA binding and RNA binding） and can also mediate protein-protein interaction. This article gives a primary report about the human ZCCHC9 gene. Protein ZCCHC9 contains four CCHC motifs and is highly conserved in humans, mice...

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Bibliographic Details
Published in:Journal of genetics and genomics 2008-08, Vol.35 (8), p.467-472
Main Authors: Zhou, Aidong, Zhou, Jianlin, Yang, Liping, Liu, Mingjun, Li, Hong, Xu, Su, Han, Mei, Zhang, Jian
Format: Article
Language:English
Subjects:
Animals
Cell Nucleus - chemistry
Cells, Cultured
Cerebral Cortex - metabolism
Gene Expression
Humans
in situ hybridization
MAP Kinase Signaling System - drug effects
Mice
Mitogen-Activated Protein Kinases - antagonists & inhibitors
Mitogen-Activated Protein Kinases - metabolism
NF-kappa B
Nuclear Proteins - genetics
Nuclear Proteins - metabolism
Nuclear Proteins - pharmacology
Polymerase Chain Reaction
Rats
real-time RT-PCR
RNA-Binding Proteins - genetics
RNA-Binding Proteins - metabolism
RNA-Binding Proteins - pharmacology
SRE
subcellular localization
ZCCHC9
ZCCHC9 gene
Zinc Fingers - genetics
Zinc Fingers - physiology
大脑皮层
生物技术
蛋白质
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Summary:The CCHC-type zinc finger motif has numerous biological activities （such as DNA binding and RNA binding） and can also mediate protein-protein interaction. This article gives a primary report about the human ZCCHC9 gene. Protein ZCCHC9 contains four CCHC motifs and is highly conserved in humans, mice, and rats. The whole eDNA sequence of the ZCCHC9 gene has been amplified by PCR and a number of plasmids have been constructed for further study. The results show that ZCCHC9 is localized in the nucleus, and especially concentrated in the nueleolus. It is highly expressed in the brain and testicles of the mouse. This has been confirmed by real-time reverse transcription polymerase chain reaction （RT-PCR）. In situ hybridization of the mouse brain indicates that ZCCHC9 is mainly expressed in the cerebral cortex. Reporter gene assay shows that ZCCHC9 suppresses the transcription activities of NF-kappa B and SRE, and may play roles in the Mitogen-Aetivated Protein Kinase （MAPK） signaling transduetion pathway.
ISSN:1673-8527
DOI:10.1016/S1673-8527(08)60064-8