Anxiolytic property of Lactuca sativa,effect on anxiety behaviour induced by novel food and height

• Published in: Asian Pacific journal of tropical medicine 2013-07, Vol.6 (7), p.532-536
• Main Authors: Harsha, SN, Anilakumar, KR
• Format: Article
• Language: English
• Subjects: Analysis of Variance; Animals; Anti-Anxiety Agents - pharmacology; Anxiety; Anxiety - drug therapy; Behavior, Animal - drug effects; Chromatography, High Pressure Liquid; Diazepam - pharmacology; Elevated; Elevated T maze test; Ethanol - pharmacology; Hyponeophagia; Lactuca; Lactuca sativa; Male; maze; Maze Learning - drug effects; Mice; Phytotherapy - methods; Plant Extracts - pharmacology; Plant Leaves; Polyphenols; Polyphenols - isolation & purification; Polyphenols - pharmacology; sativa; test
• ISSN: 1995-7645; 2352-4146
• DOI: 10.1016/S1995-7645(13)60091-7

<正>Objective:To study anxiolytic property of hydro alchohol extract and to estimate polyphenols present in the extract by HPLC.Methods:To evaluate anxiolytic properly two animal models were used viz.Elevated T maze and hyponeophagia.Diazepam(1 mg/kg body wt.) served as the standard anxiolvtic agent for all the tests.The dried extract of the plant leaf in doses of 100.200 and 400 mg/kg body weight was administered orally to mice for duration of 1? or 30 days and locomotor and anxiolytic activities were performed.Polyphenols was estimated using HPLC. Results:The HPLC analysis of the polyphenols revealed the presence chlorogenic acid,vanillin, epicatechin,caffeic acid,rutin hydrate,sinapic acid,quercetin-3-rhamnoside.p-coumeric acid and quercitin.Time spent and number of entries into the open arm was improved in 30 days treated animals than that of 15 days treated groups,200 and 400 mg/kg body weight treated group showed significant results when comparing with the control group.Conclusions:Te hydro alcohol extract rich in Polyphenols and other secondary metabolites is a potent anxiolytic agent.