Effects of recombinant adenovirus-mediated hypoxia-inducible factor-1alpha gene on proliferation and differentiation of endogenous neural stem cells in rats following intracerebral hemorrhage

To investigate the effects of adenovirus (Ad)-mediated hypoxia-inducible factor-1alpha (HIF-1α) gene on proliferation and differentiation of endogenous neural stem cells (NSCs) in rats following intracerebral hemorrhage (ICH) and the underlying mechanisms. A total of 120 specific pathogen-free, adul...

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Published in:Asian Pacific journal of tropical medicine 2013-10, Vol.6 (10), p.762-767
Main Authors: Yu, Zhen, Chen, Li-Fen, Tang, Ling, Hu, Chang-Lin
Format: Article
Language:English
Subjects:
Adenoviridae - genetics
Adenoviridae - metabolism
Adenovirus
Animals
Cell Differentiation
Cell Proliferation
Cerebral Hemorrhage - genetics
Cerebral Hemorrhage - metabolism
Cerebral Hemorrhage - physiopathology
Cerebral Hemorrhage - therapy
Endogenous neural stem cells
Gene Expression
Genetic Therapy
Humans
Hypoxia-Inducible Factor 1, alpha Subunit - genetics
Hypoxia-Inducible Factor 1, alpha Subunit - metabolism
Hypoxia-Inducible Factor 1, alpha Subunit - therapeutic use
Hypoxia-inducible factor-1alpha
Intracerebral hemorrhage
Male
Neural Stem Cells - cytology
Neural Stem Cells - metabolism
Rats
Rats, Sprague-Dawley
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Summary:To investigate the effects of adenovirus (Ad)-mediated hypoxia-inducible factor-1alpha (HIF-1α) gene on proliferation and differentiation of endogenous neural stem cells (NSCs) in rats following intracerebral hemorrhage (ICH) and the underlying mechanisms. A total of 120 specific pathogen-free, adult, male Sprague-Dawley rats were included in this study. After establishment of ICH models in rats, PBS, Ad, or Ad-HIF-1α was administered via the ischemic ventricle. On the 1st, 7th, 14th, 21st and 28th d after ICH, rat neurological deficits were scored, doublecortin (DCX) expression in the subventricular zone cells was detected by immunohistochemical staining, and 5-bromo-2'-deoxyuridine (BrdU)-, BrdU/DCX-, and BrdU/glial fibrillary acidic protein-positive cells in the subventricular zone were counted using immumofluorescence method among PBS, Ad, and Ad-HIF-1α groups. On the 7th, 14th, 21st and 28th d after ICH, neurological deficit scores in the Ad-HIF-1α group were significantly lower than in the PBS and Ad groups (P
ISSN:1995-7645
2352-4146
DOI:10.1016/S1995-7645(13)60134-0