Relationship between expression of COX-2,TNF-α,IL-6 and autoimmune-type recurrent miscarriage

• Published in: Asian Pacific journal of tropical medicine 2013-12, Vol.6 (12), p.990-994
• Main Authors: Hua, Fu, Li, Chang-Hua, Wang, Hong, Xu, Hong-Ge
• Format: Article
• Language: English
• Subjects: Abortion, Habitual - genetics; Abortion, Habitual - immunology; Abortion, Habitual - metabolism; Animal; Animal model; Animals; Autoimmune; Autoimmunity - genetics; Autoimmunity - immunology; Cyclooxygenase 2 - analysis; Cyclooxygenase 2 - blood; Cyclooxygenase 2 - genetics; Cyclooxygenase 2 - metabolism; Disease Models, Animal; Embryo, Mammalian - chemistry; Embryo, Mammalian - metabolism; Female; interface; Interleukin-6 - analysis; Interleukin-6 - blood; Interleukin-6 - genetics; Interleukin-6 - metabolism; Male; Maternal-fetal; Maternal-fetal interface; Maternal-Fetal Relations; Mice; Mice, Inbred CBA; miscarriage; Recurrent; Recurrent miscarriage; Statistics, Nonparametric; Trophoblasts - chemistry; Trophoblasts - metabolism; Tumor Necrosis Factor-alpha - analysis; Tumor Necrosis Factor-alpha - blood; Tumor Necrosis Factor-alpha - genetics; Tumor Necrosis Factor-alpha - metabolism
• ISSN: 1995-7645; 2352-4146
• DOI: 10.1016/S1995-7645(13)60178-9

Objective:To investigate the roles of COX-2,TNF-α,IL-6 in the pathogenesis of autoimmunetype recurrent spontaneous abortion(RSA).Methods:RT-PCR was used to detect the mRNA of COX-2,TNF-α.IL-6 in the trophoblast cells of murine RSA and normal pregnant models.The COX-2,TNF-α.IL-6 protein expressions were determined by using immunohistochemisry staining method.The COX-2,TNF-α,IL-6 protein expressions were determined by ELISA.Results:The embryo loss rates in experiment group was significantly higher than that in normal pregnancy control group,the expression of COX-2,TNF-α,IL-6 in the trophoblast cells of murine RSA and normal pregnant models.The expression of COX-2 in autoimmunetype recurrent spontaneous abortion was significantly lesser than in normal pregnant models.The expression of TNF-α,IL-6 in autoimmune-type recurrent spontaneous abortion was significantly higher than in normal pregnant models.There was a positively correlation between TNF-αand IL-6.There was no relationship between COX-2,TNF-αand IL-6.Conclusions:The abnormal expression of COX-2,TNF-αand IL-6 may result in RSA.