Potential of four marine-derived fungi extracts as anti-proliferative and cell death-inducing agents in seven human cancer cell lines

• Published in: Asian Pacific journal of tropical medicine 2015-10, Vol.8 (10), p.782-790
• Main Authors: Ramos, Alice Abreu, Prata-Sena, Maria, Castro-Carvalho, Bruno, Dethoup, Tida, Buttachon, Suradet, Kijjoa, Anake, Rocha, Eduardo
• Format: Article
• Language: English
• Subjects: activity; Antineoplastic; agents; Anti-proliferative; Anti-proliferative activity; Antineoplastic agents; Cell death; Cytotoxicity; Human cancer cell lines; Marine-derived fungi
• ISSN: 1995-7645; 2352-4146
• DOI: 10.1016/j.apjtm.2015.09.005

Objective:To evaluate the in vitro anticancer activity of crude ethyl acetate extracts of the culture of four marine-derived fungi Aspergillus similanensis KUFA 0013(E1),Neosartorya paulistemis KUFC 7897(E2),Neosartorya siamensis KUFA 0017(E4) and Talaromyces trachyspermus KUFC 0021(E3) on a panel of seven human cancer cell lines.Methods:Effects on cell proliferation,induction of DNA damage and cell death were assessed by MTT and clonogenic assays,comet assay and nuclear condensation assay,respectively.Results:The proliferation of HepG2,HCTl 16 and A375 cells decreased after incubation with the extracts E2 and E4.The anti-proliterative effect was confirmed by morphologic alterations and by clonogenic assay.Both extracts also induced cell death in HepG2 and HCT116 cells.Doxorubicin was used as a positive control and showed in vitro anticancer activity.Conclusions:This study demonstrated,for the first time,that extracts of Neosartorya paulistensis and Neosartorya siamensis have selective anti-proliferative and cell death activities in HepG2,HCT16 and A375 cells.The bioactivity of these extracts suggests a potential for biotechnological applications and substantiates that both should be further considered for the elucidation of the molecular targets and signal transduction pathways involved.