Anti-inflammatory evaluation and acute toxicity of three food supplements that contain Moussonia deppeana

• Published in: Asian Pacific journal of tropical medicine 2017-02, Vol.10 (2), p.134-140
• Main Author: Gabriel Alfonso Gutiérrez-Rebolledo Mariana Zuleima Pérez-González Alejandro Zamilpa María Adelina Jiménez-Arellanes
• Format: Article
• Language: English
• Subjects: Acute inflammation; Food supplements; Herbal remedies; Mexican medicinal plants; Moussonia deppeana; Traditional medicine
• ISSN: 1995-7645; 2352-4146
• DOI: 10.1016/j.apjtm.2017.01.008

Objective:To identify the anti-inflammatory activity through two murine models and in the median Lethal Dose(LD50) of three dietary supplements that contain Moussonia deppeana.Methods:The anti-inflammatory activity of three dietary supplements(Cicatrisan/GastricusR,GastinolR,and GastrovitaR) EtOH extracts was evaluated by TPA and by carrageenan murine models;also,median Lethal Dose(LD50) was determined.Verbascoside was quantified by High-Performance Liquid Chromatography.β-sitosterol,stigmasterol and the mixture of ursolic and oleanolic acids were identified in all supplements by TLC;however,none of these dietary supplements contain verbascoside.Results:For the TPA model,Cicatrisan/GastricusRgenerated a notable effect with 38.24%inhibition.While in the carrageenan model,it also exhibited noteworthy anti-inflammatory activity of ear edema with 66.39%of paw edema inhibition at 150 mg/kg,followed by GastinolR and GastrovitaR with 50%at 300 mg/kg.Finally,LD50 was >2 g/kg for all supplements,when was administered intragastrically and Body Weight（BW） gain in mice was not altered after 14 days.Conclusions:Of the three food supplements containing M.deppeana,only the EtOH extract from the Cicatrisan/GastricusRformulation（tablets） showed significant anti-inflammatory activity in both experimental models and the LD50 was > 2 g/kg.