Comparison of the Immunogenicities of HIV-1 Mutants Based on Structural Modification of env

• Published in: Virologica Sinica 2008, Vol.23 (4), p.233-246
• Main Authors: Nie, Jian-hui, Zhang, Chun-tao, Chong, Hui-hui, Wu, Xue-ling, Liu, Chun-yu, Wu, Yu, Zhao, Chen-yan, Zhang, Lin-qi, Wang, You-chun
• Format: Article
• Language: English
• Subjects: Biochemistry; Biomedical and Life Sciences; Biomedicine; Enzyme-linked immunosorbent assay; Epitopes; HIV; Human immunodeficiency virus; Human immunodeficiency virus 1; Immune response (humoral); Lymphocytes T; Medical Microbiology; Microbial Genetics and Genomics; Microbiology; Mutants; Oncology; Site-directed mutagenesis; Virology; γ-Interferon; 中和化验; 人体免疫缺损病毒; 突变异种; 艾滋病
• ISSN: 1674-0769; 1995-820X
• DOI: 10.1007/s12250-008-2949-4

Eleven env mutants were designed and generated by site-directed mutagenesis of the regions around NAb epitopes and deletions of variable regions in env. The immunogenicities of the generated mutants were evaluated using single-cycle infection neutralization assays with two pseudoviruses and IFN-γ ELISPOT. Overall, five mutants （dWt, M2, M5-2, M5-1 and dM7） induced higher neutralization activities for both pseudoviruses than plasmid Wt, while only two of the mutants （dWt and M5-2） showed significant differences （P〈0.05）. Two mutants （M2 and dM2） induced more Env-specific T cells than plasmid Wt. Statistically however, significance was only reached for mutant M2. Thus, properly modified HIV-1 Env may have the potential to induce potent cellular and humoral immune responses