Investigation of the interaction of DNA and neutral red by fluorescence spectroscopic analysis

The binding characteristics of neutral red (NR) with DNA were investigated by fluorescence spectrometry. Chemometrics approach as singular value decomposition (SVD) was used to evaluate the number of spectral species in the drug–DNA binding process, and then the intrinsic binding constant of 1.6 × 1...

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Bibliographic Details
Published in:Chinese chemical letters 2007-05, Vol.18 (5), p.569-572
Main Authors: Ni, Yong Nian, Zhong, Xue Zhi
Format: Article
Language:English
Subjects:
Binding constant
Chemometrics
DNA
Fluorescence
Neutral red
Singular value decomposition
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Summary:The binding characteristics of neutral red (NR) with DNA were investigated by fluorescence spectrometry. Chemometrics approach as singular value decomposition (SVD) was used to evaluate the number of spectral species in the drug–DNA binding process, and then the intrinsic binding constant of 1.6 × 10 4 in base pairs and the binding site number of 0.97 were obtained from the Scatchard plot.
ISSN:1001-8417
1878-5964
DOI:10.1016/j.cclet.2007.03.027